1z7k
From Proteopedia
(New page: 200px<br /><applet load="1z7k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z7k, resolution 1.90Å" /> '''Crystal Structure of...) |
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| - | [[Image:1z7k.gif|left|200px]]<br /><applet load="1z7k" size=" | + | [[Image:1z7k.gif|left|200px]]<br /><applet load="1z7k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z7k, resolution 1.90Å" /> | caption="1z7k, resolution 1.90Å" /> | ||
'''Crystal Structure of Trypsin- Ovomucoid turkey egg white inhibitor complex'''<br /> | '''Crystal Structure of Trypsin- Ovomucoid turkey egg white inhibitor complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystal structure of the complex between porcine beta-trypsin and the | + | Crystal structure of the complex between porcine beta-trypsin and the second domain of the Kazal-type ovomucoid turkey egg white trypsin inhibitor (OMTKY2) has been determined at 1.9A resolution. A peptide fragment from the first domain has been crystallized with the complex. Restrained-refinement of the structure led to an R-factor of 0.19 for the 32206 reflections. OMTKY2 exhibits the canonical Kazal-type fold with a central alpha-helix and a short two-stranded anti-parallel beta-sheet. The carbonyl carbon of the reactive site prefers trigonal geometry. The reactive site loop geometry of the inhibitor is complementary to the surface and charge of the binding site in beta-trypsin. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z7K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure | + | 1Z7K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry 1R0T. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
[[Category: Trypsin]] | [[Category: Trypsin]] | ||
| - | [[Category: Ibrahim, B | + | [[Category: Ibrahim, B Syed.]] |
[[Category: Pattabhi, V.]] | [[Category: Pattabhi, V.]] | ||
[[Category: di-nag]] | [[Category: di-nag]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:51 2008'' |
Revision as of 14:12, 21 February 2008
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Crystal Structure of Trypsin- Ovomucoid turkey egg white inhibitor complex
Overview
Crystal structure of the complex between porcine beta-trypsin and the second domain of the Kazal-type ovomucoid turkey egg white trypsin inhibitor (OMTKY2) has been determined at 1.9A resolution. A peptide fragment from the first domain has been crystallized with the complex. Restrained-refinement of the structure led to an R-factor of 0.19 for the 32206 reflections. OMTKY2 exhibits the canonical Kazal-type fold with a central alpha-helix and a short two-stranded anti-parallel beta-sheet. The carbonyl carbon of the reactive site prefers trigonal geometry. The reactive site loop geometry of the inhibitor is complementary to the surface and charge of the binding site in beta-trypsin.
About this Structure
1Z7K is a Protein complex structure of sequences from Meleagris gallopavo and Sus scrofa. This structure supersedes the now removed PDB entry 1R0T. Active as Trypsin, with EC number 3.4.21.4 Full crystallographic information is available from OCA.
Reference
Crystal structure of trypsin-turkey egg white inhibitor complex., Ibrahim BS, Pattabhi V, Biochem Biophys Res Commun. 2004 Jan 2;313(1):8-16. PMID:14672690
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