1z88
From Proteopedia
(New page: 200px<br /><applet load="1z88" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z88, resolution 2.1Å" /> '''Crystal structure of ...) |
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| - | [[Image:1z88.gif|left|200px]]<br /><applet load="1z88" size=" | + | [[Image:1z88.gif|left|200px]]<br /><applet load="1z88" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z88, resolution 2.1Å" /> | caption="1z88, resolution 2.1Å" /> | ||
'''Crystal structure of Lys154Arg mutant of mature AphA of S. typhimurium'''<br /> | '''Crystal structure of Lys154Arg mutant of mature AphA of S. typhimurium'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was | + | The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z88 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http:// | + | 1Z88 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z88 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Gupta, G | + | [[Category: Gupta, G D.]] |
[[Category: Kumar, V.]] | [[Category: Kumar, V.]] | ||
| - | [[Category: Makde, R | + | [[Category: Makde, R D.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: class-b bacterial acid phosphatase]] | [[Category: class-b bacterial acid phosphatase]] | ||
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[[Category: metalloenzyme]] | [[Category: metalloenzyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:00 2008'' |
Revision as of 14:13, 21 February 2008
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Crystal structure of Lys154Arg mutant of mature AphA of S. typhimurium
Overview
The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.
About this Structure
1Z88 is a Single protein structure of sequence from Salmonella typhimurium with as ligand. Active as Acid phosphatase, with EC number 3.1.3.2 Full crystallographic information is available from OCA.
Reference
Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135
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