1zb1
From Proteopedia
(New page: 200px<br /><applet load="1zb1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zb1, resolution 1.95Å" /> '''Structure basis for ...) |
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| - | [[Image:1zb1.gif|left|200px]]<br /><applet load="1zb1" size=" | + | [[Image:1zb1.gif|left|200px]]<br /><applet load="1zb1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1zb1, resolution 1.95Å" /> | caption="1zb1, resolution 1.95Å" /> | ||
'''Structure basis for endosomal targeting by the Bro1 domain'''<br /> | '''Structure basis for endosomal targeting by the Bro1 domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Proteins delivered to the lysosome or the yeast vacuole via late endosomes | + | Proteins delivered to the lysosome or the yeast vacuole via late endosomes are sorted by the ESCRT complexes and by associated proteins, including Alix and its yeast homolog Bro1. Alix, Bro1, and several other late endosomal proteins share a conserved 160 residue Bro1 domain whose boundaries, structure, and function have not been characterized. The crystal structure of the Bro1 domain of Bro1 reveals a folded core of 367 residues. The extended Bro1 domain is necessary and sufficient for binding to the ESCRT-III subunit Snf7 and for the recruitment of Bro1 to late endosomes. The structure resembles a boomerang with its concave face filled in and contains a triple tetratricopeptide repeat domain as a substructure. Snf7 binds to a conserved hydrophobic patch on Bro1 that is required for protein complex formation and for the protein-sorting function of Bro1. These results define a conserved mechanism whereby Bro1 domain-containing proteins are targeted to endosomes by Snf7 and its orthologs. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZB1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1ZB1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Beach, B | + | [[Category: Beach, B M.]] |
[[Category: Hierro, A.]] | [[Category: Hierro, A.]] | ||
| - | [[Category: Hurley, J | + | [[Category: Hurley, J H.]] |
[[Category: Kim, J.]] | [[Category: Kim, J.]] | ||
[[Category: Odorizzi, G.]] | [[Category: Odorizzi, G.]] | ||
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[[Category: trafficking]] | [[Category: trafficking]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:52 2008'' |
Revision as of 14:14, 21 February 2008
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Structure basis for endosomal targeting by the Bro1 domain
Overview
Proteins delivered to the lysosome or the yeast vacuole via late endosomes are sorted by the ESCRT complexes and by associated proteins, including Alix and its yeast homolog Bro1. Alix, Bro1, and several other late endosomal proteins share a conserved 160 residue Bro1 domain whose boundaries, structure, and function have not been characterized. The crystal structure of the Bro1 domain of Bro1 reveals a folded core of 367 residues. The extended Bro1 domain is necessary and sufficient for binding to the ESCRT-III subunit Snf7 and for the recruitment of Bro1 to late endosomes. The structure resembles a boomerang with its concave face filled in and contains a triple tetratricopeptide repeat domain as a substructure. Snf7 binds to a conserved hydrophobic patch on Bro1 that is required for protein complex formation and for the protein-sorting function of Bro1. These results define a conserved mechanism whereby Bro1 domain-containing proteins are targeted to endosomes by Snf7 and its orthologs.
About this Structure
1ZB1 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural basis for endosomal targeting by the Bro1 domain., Kim J, Sitaraman S, Hierro A, Beach BM, Odorizzi G, Hurley JH, Dev Cell. 2005 Jun;8(6):937-47. PMID:15935782
Page seeded by OCA on Thu Feb 21 16:13:52 2008
Categories: Saccharomyces cerevisiae | Single protein | Beach, B M. | Hierro, A. | Hurley, J H. | Kim, J. | Odorizzi, G. | Sitaraman, S. | Aip1 | Bro1 | Bro1 domain | Snf7 | Trafficking
