1zla
From Proteopedia
(New page: 200px<br /><applet load="1zla" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zla, resolution 2.9Å" /> '''X-ray Structure of a ...) |
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| - | [[Image:1zla.gif|left|200px]]<br /><applet load="1zla" size=" | + | [[Image:1zla.gif|left|200px]]<br /><applet load="1zla" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1zla, resolution 2.9Å" /> | caption="1zla, resolution 2.9Å" /> | ||
'''X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core'''<br /> | '''X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear | + | Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through the folded region of histones H2A-H2B. The same LANA residues were required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe the 2.9-angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZLA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Expression_vector_pcy215 Expression vector pcy215], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http:// | + | 1ZLA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Expression_vector_pcy215 Expression vector pcy215], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
| - | [[Category: Barbera, A | + | [[Category: Barbera, A J.]] |
| - | [[Category: Chodaparambil, J | + | [[Category: Chodaparambil, J V.]] |
| - | [[Category: Kaye, K | + | [[Category: Kaye, K M.]] |
[[Category: Luger, K.]] | [[Category: Luger, K.]] | ||
[[Category: chromatin]] | [[Category: chromatin]] | ||
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[[Category: protein/protein interaction]] | [[Category: protein/protein interaction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:40 2008'' |
Revision as of 14:16, 21 February 2008
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X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core
Overview
Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through the folded region of histones H2A-H2B. The same LANA residues were required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe the 2.9-angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency.
About this Structure
1ZLA is a Protein complex structure of sequences from Expression vector pcy215, Homo sapiens and Xenopus laevis. Full crystallographic information is available from OCA.
Reference
The nucleosomal surface as a docking station for Kaposi's sarcoma herpesvirus LANA., Barbera AJ, Chodaparambil JV, Kelley-Clarke B, Joukov V, Walter JC, Luger K, Kaye KM, Science. 2006 Feb 10;311(5762):856-61. PMID:16469929
Page seeded by OCA on Thu Feb 21 16:16:40 2008
Categories: Expression vector pcy215 | Homo sapiens | Protein complex | Xenopus laevis | Barbera, A J. | Chodaparambil, J V. | Kaye, K M. | Luger, K. | Chromatin | Kaposi's sarcoma herpes virus (kshv) | Latency associated nuclear antigen (lana) | Nucleosome core particle | Protein/protein interaction
