1znn
From Proteopedia
(New page: 200px<br /><applet load="1znn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1znn, resolution 2.20Å" /> '''Structure of the syn...) |
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| - | [[Image:1znn.gif|left|200px]]<br /><applet load="1znn" size=" | + | [[Image:1znn.gif|left|200px]]<br /><applet load="1znn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1znn, resolution 2.20Å" /> | caption="1znn, resolution 2.20Å" /> | ||
'''Structure of the synthase subunit of PLP synthase'''<br /> | '''Structure of the synthase subunit of PLP synthase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pyridoxal 5'-phosphate (PLP, vitamin B6), a cofactor in many enzymatic | + | Pyridoxal 5'-phosphate (PLP, vitamin B6), a cofactor in many enzymatic reactions, has two distinct biosynthetic routes, which do not coexist in any organism. Two proteins, known as PdxS and PdxT, together form a PLP synthase in plants, fungi, archaea, and some eubacteria. PLP synthase is a heteromeric glutamine amidotransferase in which PdxT produces ammonia from glutamine and PdxS combines ammonia with five- and three-carbon phosphosugars to form PLP. In the 2.2-A crystal structure, PdxS is a cylindrical dodecamer of subunits having the classic (beta/alpha)8 barrel fold. PdxS subunits form two hexameric rings with the active sites positioned on the inside. The hexamer and dodecamer forms coexist in solution. A novel phosphate-binding site is suggested by bound sulfate. The sulfate and another bound molecule, methyl pentanediol, were used to model the substrate ribulose 5-phosphate, and to propose catalytic roles for residues in the active site. The distribution of conserved surfaces in the PdxS dodecamer was used to predict a docking site for the glutaminase partner, PdxT. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with SO4 and MRD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ZNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=MRD:'>MRD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZNN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Belitsky, B | + | [[Category: Belitsky, B R.]] |
| - | [[Category: Burgner, J | + | [[Category: Burgner, J W.]] |
[[Category: Harms, E.]] | [[Category: Harms, E.]] | ||
| - | [[Category: Smith, J | + | [[Category: Smith, J L.]] |
[[Category: Zhu, J.]] | [[Category: Zhu, J.]] | ||
[[Category: MRD]] | [[Category: MRD]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:17:21 2008'' |
Revision as of 14:17, 21 February 2008
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Structure of the synthase subunit of PLP synthase
Overview
Pyridoxal 5'-phosphate (PLP, vitamin B6), a cofactor in many enzymatic reactions, has two distinct biosynthetic routes, which do not coexist in any organism. Two proteins, known as PdxS and PdxT, together form a PLP synthase in plants, fungi, archaea, and some eubacteria. PLP synthase is a heteromeric glutamine amidotransferase in which PdxT produces ammonia from glutamine and PdxS combines ammonia with five- and three-carbon phosphosugars to form PLP. In the 2.2-A crystal structure, PdxS is a cylindrical dodecamer of subunits having the classic (beta/alpha)8 barrel fold. PdxS subunits form two hexameric rings with the active sites positioned on the inside. The hexamer and dodecamer forms coexist in solution. A novel phosphate-binding site is suggested by bound sulfate. The sulfate and another bound molecule, methyl pentanediol, were used to model the substrate ribulose 5-phosphate, and to propose catalytic roles for residues in the active site. The distribution of conserved surfaces in the PdxS dodecamer was used to predict a docking site for the glutaminase partner, PdxT.
About this Structure
1ZNN is a Protein complex structure of sequences from Geobacillus stearothermophilus with and as ligands. Full crystallographic information is available from OCA.
Reference
A new arrangement of (beta/alpha)8 barrels in the synthase subunit of PLP synthase., Zhu J, Burgner JW, Harms E, Belitsky BR, Smith JL, J Biol Chem. 2005 Jul 29;280(30):27914-23. Epub 2005 May 23. PMID:15911615
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