1ztm
From Proteopedia
(New page: 200px<br /><applet load="1ztm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ztm, resolution 3.05Å" /> '''Structure of the Unc...) |
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| - | [[Image:1ztm.gif|left|200px]]<br /><applet load="1ztm" size=" | + | [[Image:1ztm.gif|left|200px]]<br /><applet load="1ztm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ztm, resolution 3.05Å" /> | caption="1ztm, resolution 3.05Å" /> | ||
'''Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein'''<br /> | '''Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Class I viral fusion proteins share common mechanistic and structural | + | Class I viral fusion proteins share common mechanistic and structural features but little sequence similarity. Structural insights into the protein conformational changes associated with membrane fusion are based largely on studies of the influenza virus hemagglutinin in pre- and postfusion conformations. Here, we present the crystal structure of the secreted, uncleaved ectodomain of the paramyxovirus, human parainfluenza virus 3 fusion (F) protein, a member of the class I viral fusion protein group. The secreted human parainfluenza virus 3 F forms a trimer with distinct head, neck, and stalk regions. Unexpectedly, the structure reveals a six-helix bundle associated with the postfusion form of F, suggesting that the anchor-minus ectodomain adopts a conformation largely similar to the postfusion state. The transmembrane anchor domains of F may therefore profoundly influence the folding energetics that establish and maintain a metastable, prefusion state. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZTM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_parainfluenza_virus_4 Human parainfluenza virus 4] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1ZTM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_parainfluenza_virus_4 Human parainfluenza virus 4] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Human parainfluenza virus 4]] | [[Category: Human parainfluenza virus 4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Jardetzky, T | + | [[Category: Jardetzky, T S.]] |
| - | [[Category: Lamb, R | + | [[Category: Lamb, R A.]] |
| - | [[Category: Paterson, R | + | [[Category: Paterson, R G.]] |
[[Category: Wen, X.]] | [[Category: Wen, X.]] | ||
| - | [[Category: Yin, H | + | [[Category: Yin, H S.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: 6-helix bundle]] | [[Category: 6-helix bundle]] | ||
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[[Category: trimer]] | [[Category: trimer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:18:57 2008'' |
Revision as of 14:18, 21 February 2008
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Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein
Overview
Class I viral fusion proteins share common mechanistic and structural features but little sequence similarity. Structural insights into the protein conformational changes associated with membrane fusion are based largely on studies of the influenza virus hemagglutinin in pre- and postfusion conformations. Here, we present the crystal structure of the secreted, uncleaved ectodomain of the paramyxovirus, human parainfluenza virus 3 fusion (F) protein, a member of the class I viral fusion protein group. The secreted human parainfluenza virus 3 F forms a trimer with distinct head, neck, and stalk regions. Unexpectedly, the structure reveals a six-helix bundle associated with the postfusion form of F, suggesting that the anchor-minus ectodomain adopts a conformation largely similar to the postfusion state. The transmembrane anchor domains of F may therefore profoundly influence the folding energetics that establish and maintain a metastable, prefusion state.
About this Structure
1ZTM is a Single protein structure of sequence from Human parainfluenza virus 4 with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the uncleaved ectodomain of the paramyxovirus (hPIV3) fusion protein., Yin HS, Paterson RG, Wen X, Lamb RA, Jardetzky TS, Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9288-93. Epub 2005 Jun 17. PMID:15964978
Page seeded by OCA on Thu Feb 21 16:18:57 2008
