2c12
From Proteopedia
(New page: 200px<br /> <applet load="2c12" size="450" color="white" frame="true" align="right" spinBox="true" caption="2c12, resolution 2.07Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2C12 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]] with SPM, FAD, PE4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C12 OCA]]. | + | 2C12 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]] with SPM, FAD, PE4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C12 OCA]]. |
==Reference== | ==Reference== | ||
| Line 30: | Line 30: | ||
[[Category: nitroalkane]] | [[Category: nitroalkane]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:12:24 2007'' |
Revision as of 12:07, 30 October 2007
|
CRYSTAL STRUCTURE OF NITROALKANE OXIDASE IN COMPLEX WITH SPERMINE, A COMPETITIVE INHIBITOR
Overview
Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation, of neutral nitroalkanes to the corresponding aldehydes or ketones with the, production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the, acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA, substrates. We present the 2.2 A resolution crystal structure of NAO, trapped during the turnover of nitroethane as a covalent N5-FAD adduct, (ES*). The homotetrameric structure of ES* was solved by MAD phasing with, 52 Se-Met sites in an orthorhombic space group. The electron density for, the N5-(2-nitrobutyl)-1,5-dihydro-FAD covalent intermediate is clearly, resolved. The structure of ES was used to solve the crystal structure of, oxidized NAO at 2.07 A resolution. The c axis for the ... [(full description)]
About this Structure
2C12 is a [Single protein] structure of sequence from [Fusarium oxysporum] with SPM, FAD, PE4 and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover., Nagpal A, Valley MP, Fitzpatrick PF, Orville AM, Biochemistry. 2006 Jan 31;45(4):1138-50. PMID:16430210
Page seeded by OCA on Tue Oct 30 14:12:24 2007
Categories: Fusarium oxysporum | Single protein | Fitzpatrick, P.F. | Nagpal, A. | Orville, A.M. | Valley, M.P. | FAD | GOL | PE4 | SPM | Acyl-coa dehydrogenase | Fad | Flavoenzyme | Flavoprotein | Inhibitor | Long cell edge | Nitroalkane
