2a2g
From Proteopedia
(New page: 200px<br /><applet load="2a2g" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a2g, resolution 2.90Å" /> '''THE CRYSTAL STRUCTUR...) |
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| - | [[Image:2a2g.gif|left|200px]]<br /><applet load="2a2g" size=" | + | [[Image:2a2g.gif|left|200px]]<br /><applet load="2a2g" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2a2g, resolution 2.90Å" /> | caption="2a2g, resolution 2.90Å" /> | ||
'''THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.'''<br /> | '''THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult | + | Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult male rats. The structure of a monoclinic crystal form of A2U was reported in 1992 [Bocskei et al. (1992). Nature (London), 360, 186-188]. The structures of an orthorhombic crystal form of A2U at 2. 5 A resolution (refined to an R factor of 0.248; Rfree = 0.264) and of a complex between A2U and d-limonene 1,2-epoxide (DLO) at 2.9 A resolution (R factor = 0.248; Rfree = 0.260) are presented here. DLO is one of a diverse group of chemicals which cause a male rat-specific renal carcinogenesis called hyaline-droplet nephropathy. The rate-determining step in the development of this disorder is the binding of the toxin to A2U. Comparison of the cavities in A2U and in the corresponding mouse urinary protein (MUP) reveal that the former is tailor-made for small oval hydrophobic ligands such as DLO. The cavity in MUP is more shallow and elongated and cannot easily accommodate such ligands. |
==About this Structure== | ==About this Structure== | ||
| - | 2A2G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with LEO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2A2G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=LEO:'>LEO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A2G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chaudhuri, B | + | [[Category: Chaudhuri, B N.]] |
| - | [[Category: Jones, T | + | [[Category: Jones, T A.]] |
| - | [[Category: Kleywegt, G | + | [[Category: Kleywegt, G J.]] |
[[Category: LEO]] | [[Category: LEO]] | ||
[[Category: lipid-binding protein]] | [[Category: lipid-binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:54 2008'' |
Revision as of 14:22, 21 February 2008
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THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.
Overview
Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult male rats. The structure of a monoclinic crystal form of A2U was reported in 1992 [Bocskei et al. (1992). Nature (London), 360, 186-188]. The structures of an orthorhombic crystal form of A2U at 2. 5 A resolution (refined to an R factor of 0.248; Rfree = 0.264) and of a complex between A2U and d-limonene 1,2-epoxide (DLO) at 2.9 A resolution (R factor = 0.248; Rfree = 0.260) are presented here. DLO is one of a diverse group of chemicals which cause a male rat-specific renal carcinogenesis called hyaline-droplet nephropathy. The rate-determining step in the development of this disorder is the binding of the toxin to A2U. Comparison of the cavities in A2U and in the corresponding mouse urinary protein (MUP) reveal that the former is tailor-made for small oval hydrophobic ligands such as DLO. The cavity in MUP is more shallow and elongated and cannot easily accommodate such ligands.
About this Structure
2A2G is a Single protein structure of sequence from Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.
Reference
The structures of alpha 2u-globulin and its complex with a hyaline droplet inducer., Chaudhuri BN, Kleywegt GJ, Bjorkman J, Lehman-McKeeman LD, Oliver JD, Jones TA, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):753-62. PMID:10089305
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