2a6r
From Proteopedia
(New page: 200px<br /><applet load="2a6r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a6r, resolution 2.05Å" /> '''Crystal structure of...) |
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| - | [[Image:2a6r.gif|left|200px]]<br /><applet load="2a6r" size=" | + | [[Image:2a6r.gif|left|200px]]<br /><applet load="2a6r" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2a6r, resolution 2.05Å" /> | caption="2a6r, resolution 2.05Å" /> | ||
'''Crystal structure of YoeB under PEG condition'''<br /> | '''Crystal structure of YoeB under PEG condition'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The eubacterial chromosome encodes various addiction modules that control | + | The eubacterial chromosome encodes various addiction modules that control global levels of translation through RNA degradation. Crystal structures of the Escherichia coli YefM2 (antitoxin)-YoeB (toxin) complex and the free YoeB toxin have been determined. The structure of the heterotrimeric complex reveals an asymmetric disorder-to-order recognition strategy, in which one C terminus of the YefM homodimer exclusively interacts with an atypical microbial ribonuclease (RNase) fold of YoeB. Comparison with the YefM-free YoeB structure indicates a conformational rearrangement of the RNase catalytic site of YoeB, induced by interaction with YefM. Complementary biochemical experiments demonstrate that the YoeB toxin has an in vitro RNase activity that preferentially cleaves at the 3' end of purine ribonucleotides. |
==About this Structure== | ==About this Structure== | ||
| - | 2A6R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2A6R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: yoeb]] | [[Category: yoeb]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:08 2008'' |
Revision as of 14:24, 21 February 2008
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Crystal structure of YoeB under PEG condition
Overview
The eubacterial chromosome encodes various addiction modules that control global levels of translation through RNA degradation. Crystal structures of the Escherichia coli YefM2 (antitoxin)-YoeB (toxin) complex and the free YoeB toxin have been determined. The structure of the heterotrimeric complex reveals an asymmetric disorder-to-order recognition strategy, in which one C terminus of the YefM homodimer exclusively interacts with an atypical microbial ribonuclease (RNase) fold of YoeB. Comparison with the YefM-free YoeB structure indicates a conformational rearrangement of the RNase catalytic site of YoeB, induced by interaction with YefM. Complementary biochemical experiments demonstrate that the YoeB toxin has an in vitro RNase activity that preferentially cleaves at the 3' end of purine ribonucleotides.
About this Structure
2A6R is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin., Kamada K, Hanaoka F, Mol Cell. 2005 Aug 19;19(4):497-509. PMID:16109374
Page seeded by OCA on Thu Feb 21 16:24:08 2008
Categories: Escherichia coli | Single protein | Hanaoka, F. | Kamada, K. | MG | Addiction modules | Antitoxin | Inhibitor | Rnase | Toxin | Yefm | Yoeb
