2c13
From Proteopedia
(New page: 200px<br /> <applet load="2c13" size="450" color="white" frame="true" align="right" spinBox="true" caption="2c13, resolution 2.150Å" /> '''5-HYDROXY-LEVULINI...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2C13 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with MG, CL, K, PGE and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C13 OCA]]. | + | 2C13 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with MG, CL, K, PGE and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C13 OCA]]. |
==Reference== | ==Reference== | ||
Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors., Frere F, Nentwich M, Gacond S, Heinz DW, Neier R, Frankenberg-Dinkel N, Biochemistry. 2006 Jul 11;45(27):8243-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16819823 16819823] | Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors., Frere F, Nentwich M, Gacond S, Heinz DW, Neier R, Frankenberg-Dinkel N, Biochemistry. 2006 Jul 11;45(27):8243-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16819823 16819823] | ||
| + | [[Category: Porphobilinogen synthase]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: porphyrin biosynthesis]] | [[Category: porphyrin biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:13:14 2007'' |
Revision as of 12:08, 30 October 2007
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5-HYDROXY-LEVULINIC ACID BOUND TO PORPHOBILINOGEN SYNTHASE FROM PSEUDOMONAS AERUGINOSA
Overview
Porphobilinogen synthase catalyzes the first committed step of the, tetrapyrrole biosynthesis pathway. In an aldol-like condensation, two, molecules of 5-aminolevulinic acid (ALA) form the first pyrrole, porphobilinogen. Newly synthesized analogues of a reaction intermediate of, porphobilinogen synthase have been employed in studying the active site, and the catalytic mechanism of this early enzyme of tetrapyrrole, biosynthesis. This study combines structural and kinetic evaluation of the, inhibition potency of these inhibitors. In addition, one of the determined, protein structures provides for the first time structural evidence of a, magnesium ion in the active site. From these results, we can corroborate, an earlier postulated enzymatic mechanism that starts with formation of a, C-C ... [(full description)]
About this Structure
2C13 is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with MG, CL, K, PGE and PEG as [ligands]. Active as [Porphobilinogen synthase], with EC number [4.2.1.24]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors., Frere F, Nentwich M, Gacond S, Heinz DW, Neier R, Frankenberg-Dinkel N, Biochemistry. 2006 Jul 11;45(27):8243-53. PMID:16819823
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