2afj
From Proteopedia
(New page: 200px<br /><applet load="2afj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2afj" /> '''SPRY domain-containing SOCS box protein 2 (S...) |
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| - | [[Image:2afj.gif|left|200px]]<br /><applet load="2afj" size=" | + | [[Image:2afj.gif|left|200px]]<br /><applet load="2afj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2afj" /> | caption="2afj" /> | ||
'''SPRY domain-containing SOCS box protein 2 (SSB-2)'''<br /> | '''SPRY domain-containing SOCS box protein 2 (SSB-2)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The four mammalian SPRY domain-containing SOCS box proteins (SSB-1 to | + | The four mammalian SPRY domain-containing SOCS box proteins (SSB-1 to SSB-4) are characterized by a C-terminal SOCS box and a central SPRY domain. We have determined the first SPRY-domain structure, as part of SSB-2, by NMR. This domain adopts a novel fold consisting of a beta-sandwich structure formed by two four-stranded antiparallel beta-sheets with a unique topology. We demonstrate that SSB-1, SSB-2 and SSB-4, but not SSB-3, bind prostate apoptosis response protein-4 (Par-4). Mutational analysis of SSB-2 loop regions identified conserved structural determinants for its interaction with Par-4 and the hepatocyte growth factor receptor, c-Met. Mutations in analogous loop regions of pyrin and midline-1 SPRY domains have been shown to cause Mediterranean fever and Opitz syndrome, respectively. Our findings provide a template for SPRY-domain structure and an insight into the mechanism of SPRY-protein interaction. |
==About this Structure== | ==About this Structure== | ||
| - | 2AFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 2AFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Babon, J | + | [[Category: Babon, J J.]] |
| - | [[Category: Masters, S | + | [[Category: Masters, S L.]] |
| - | [[Category: Nicholson, S | + | [[Category: Nicholson, S E.]] |
| - | [[Category: Nicola, N | + | [[Category: Nicola, N A.]] |
| - | [[Category: Norton, R | + | [[Category: Norton, R S.]] |
| - | [[Category: Palmer, K | + | [[Category: Palmer, K R.]] |
| - | [[Category: Smith, B | + | [[Category: Smith, B J.]] |
| - | [[Category: Willson, T | + | [[Category: Willson, T A.]] |
[[Category: Yao, S.]] | [[Category: Yao, S.]] | ||
| - | [[Category: Zhang, J | + | [[Category: Zhang, J G.]] |
[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:26:53 2008'' |
Revision as of 14:26, 21 February 2008
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SPRY domain-containing SOCS box protein 2 (SSB-2)
Overview
The four mammalian SPRY domain-containing SOCS box proteins (SSB-1 to SSB-4) are characterized by a C-terminal SOCS box and a central SPRY domain. We have determined the first SPRY-domain structure, as part of SSB-2, by NMR. This domain adopts a novel fold consisting of a beta-sandwich structure formed by two four-stranded antiparallel beta-sheets with a unique topology. We demonstrate that SSB-1, SSB-2 and SSB-4, but not SSB-3, bind prostate apoptosis response protein-4 (Par-4). Mutational analysis of SSB-2 loop regions identified conserved structural determinants for its interaction with Par-4 and the hepatocyte growth factor receptor, c-Met. Mutations in analogous loop regions of pyrin and midline-1 SPRY domains have been shown to cause Mediterranean fever and Opitz syndrome, respectively. Our findings provide a template for SPRY-domain structure and an insight into the mechanism of SPRY-protein interaction.
About this Structure
2AFJ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues., Masters SL, Yao S, Willson TA, Zhang JG, Palmer KR, Smith BJ, Babon JJ, Nicola NA, Norton RS, Nicholson SE, Nat Struct Mol Biol. 2006 Jan;13(1):77-84. Epub 2005 Dec 20. PMID:16369487
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