2afp
From Proteopedia
(New page: 200px<br /><applet load="2afp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2afp" /> '''THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE...) |
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'''THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY'''<br /> | '''THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A recombinant form of the sea raven type II antifreeze protein (SRAFP) has | + | A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H- inverted question mark15N inverted question mark heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%. |
==About this Structure== | ==About this Structure== | ||
| - | 2AFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hemitripterus_americanus Hemitripterus americanus]. Full crystallographic information is available from [http:// | + | 2AFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hemitripterus_americanus Hemitripterus americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Hemitripterus americanus]] | [[Category: Hemitripterus americanus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Daugulis, A | + | [[Category: Daugulis, A J.]] |
| - | [[Category: Davies, P | + | [[Category: Davies, P L.]] |
[[Category: Gronwald, W.]] | [[Category: Gronwald, W.]] | ||
[[Category: Lix, B.]] | [[Category: Lix, B.]] | ||
| - | [[Category: Loewen, M | + | [[Category: Loewen, M C.]] |
| - | [[Category: Sonnichsen, F | + | [[Category: Sonnichsen, F D.]] |
| - | [[Category: Sykes, B | + | [[Category: Sykes, B D.]] |
[[Category: c-type lectin]] | [[Category: c-type lectin]] | ||
[[Category: recombinant sea raven protein]] | [[Category: recombinant sea raven protein]] | ||
[[Category: solution backbone fold]] | [[Category: solution backbone fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:04 2008'' |
Revision as of 14:27, 21 February 2008
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THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY
Overview
A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H- inverted question mark15N inverted question mark heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%.
About this Structure
2AFP is a Single protein structure of sequence from Hemitripterus americanus. Full crystallographic information is available from OCA.
Reference
The solution structure of type II antifreeze protein reveals a new member of the lectin family., Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sonnichsen FD, Davies PL, Sykes BD, Biochemistry. 1998 Apr 7;37(14):4712-21. PMID:9537986
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