2ai1
From Proteopedia
(New page: 200px<br /><applet load="2ai1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ai1, resolution 2.00Å" /> '''Purine nucleoside ph...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2ai1.gif|left|200px]]<br /><applet load="2ai1" size=" | + | [[Image:2ai1.gif|left|200px]]<br /><applet load="2ai1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ai1, resolution 2.00Å" /> | caption="2ai1, resolution 2.00Å" /> | ||
'''Purine nucleoside phosphorylase from calf spleen'''<br /> | '''Purine nucleoside phosphorylase from calf spleen'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In an effort to develop potent multisubstrate-analog inhibitors of purine | + | In an effort to develop potent multisubstrate-analog inhibitors of purine nucleoside phosphorylase (PNP), three nucleoside phosphonates were designed utilizing structural information from the previously reported structures of complexes of bovine PNP with substrates and products. The nucleoside phosphonates contain an acetal linkage at the O2' and O3' positions and a two-C-atom spacer between the ribose and phosphate moieties. The linkage enables the compounds to simultaneously occupy the purine-, ribose- and phosphate-binding sites. The chemical syntheses, inhibition profiles and structural characterization of these novel multisubstrate analog inhibitors with bovine PNP are described. |
==About this Structure== | ==About this Structure== | ||
| - | 2AI1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG, ZN, 144 and P1G as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] Full crystallographic information is available from [http:// | + | 2AI1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=144:'>144</scene> and <scene name='pdbligand=P1G:'>P1G</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AI1 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Purine-nucleoside phosphorylase]] | [[Category: Purine-nucleoside phosphorylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ealick, S | + | [[Category: Ealick, S E.]] |
[[Category: Ganem, B.]] | [[Category: Ganem, B.]] | ||
[[Category: Li, Y.]] | [[Category: Li, Y.]] | ||
| - | [[Category: Toms, A | + | [[Category: Toms, A V.]] |
[[Category: Wang, W.]] | [[Category: Wang, W.]] | ||
[[Category: 144]] | [[Category: 144]] | ||
| Line 26: | Line 26: | ||
[[Category: purine nucleoside phosphorylase]] | [[Category: purine nucleoside phosphorylase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:40 2008'' |
Revision as of 14:27, 21 February 2008
|
Purine nucleoside phosphorylase from calf spleen
Overview
In an effort to develop potent multisubstrate-analog inhibitors of purine nucleoside phosphorylase (PNP), three nucleoside phosphonates were designed utilizing structural information from the previously reported structures of complexes of bovine PNP with substrates and products. The nucleoside phosphonates contain an acetal linkage at the O2' and O3' positions and a two-C-atom spacer between the ribose and phosphate moieties. The linkage enables the compounds to simultaneously occupy the purine-, ribose- and phosphate-binding sites. The chemical syntheses, inhibition profiles and structural characterization of these novel multisubstrate analog inhibitors with bovine PNP are described.
About this Structure
2AI1 is a Single protein structure of sequence from Bos taurus with , , and as ligands. Active as Purine-nucleoside phosphorylase, with EC number 2.4.2.1 Full crystallographic information is available from OCA.
Reference
Novel multisubstrate inhibitors of mammalian purine nucleoside phosphorylase., Toms AV, Wang W, Li Y, Ganem B, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1449-58. Epub 2005, Oct 19. PMID:16239721
Page seeded by OCA on Thu Feb 21 16:27:40 2008
