2au3
From Proteopedia
(New page: 200px<br /><applet load="2au3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2au3, resolution 2.00Å" /> '''Crystal Structure of...) |
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| - | [[Image:2au3.gif|left|200px]]<br /><applet load="2au3" size=" | + | [[Image:2au3.gif|left|200px]]<br /><applet load="2au3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2au3, resolution 2.00Å" /> | caption="2au3, resolution 2.00Å" /> | ||
'''Crystal Structure of the Aquifex aeolicus primase (Zinc Binding and RNA Polymerase Domains)'''<br /> | '''Crystal Structure of the Aquifex aeolicus primase (Zinc Binding and RNA Polymerase Domains)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The coordination of primase function within the replisome is an essential | + | The coordination of primase function within the replisome is an essential but poorly understood feature of lagging strand synthesis. By using crystallography and small-angle X-ray scattering (SAXS), we show that functional elements of bacterial primase transition between two dominant conformations: an extended form that uncouples a regulatory domain from its associated RNA polymerase core and a compact state that sequesters the regulatory region from the site of primer synthesis. FRET studies and priming assays reveal that the regulatory domain of one primase subunit productively associates with nucleic acid that is bound to the polymerase domain of a second protomer in trans. This intersubunit interaction allows primase to select initiation sites on template DNA and implicates the regulatory domain as a "molecular brake" that restricts primer length. Our data suggest that the replisome may cooperatively use multiple primases and this conformational switch to control initiation frequency, processivity, and ultimately, Okazaki fragment synthesis. |
==About this Structure== | ==About this Structure== | ||
| - | 2AU3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2AU3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AU3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berger, J | + | [[Category: Berger, J M.]] |
| - | [[Category: Corn, J | + | [[Category: Corn, J E.]] |
| - | [[Category: Hura, G | + | [[Category: Hura, G L.]] |
| - | [[Category: Pease, P | + | [[Category: Pease, P J.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: dna replication]] | [[Category: dna replication]] | ||
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[[Category: zinc ribbon]] | [[Category: zinc ribbon]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:06 2008'' |
Revision as of 14:31, 21 February 2008
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Crystal Structure of the Aquifex aeolicus primase (Zinc Binding and RNA Polymerase Domains)
Overview
The coordination of primase function within the replisome is an essential but poorly understood feature of lagging strand synthesis. By using crystallography and small-angle X-ray scattering (SAXS), we show that functional elements of bacterial primase transition between two dominant conformations: an extended form that uncouples a regulatory domain from its associated RNA polymerase core and a compact state that sequesters the regulatory region from the site of primer synthesis. FRET studies and priming assays reveal that the regulatory domain of one primase subunit productively associates with nucleic acid that is bound to the polymerase domain of a second protomer in trans. This intersubunit interaction allows primase to select initiation sites on template DNA and implicates the regulatory domain as a "molecular brake" that restricts primer length. Our data suggest that the replisome may cooperatively use multiple primases and this conformational switch to control initiation frequency, processivity, and ultimately, Okazaki fragment synthesis.
About this Structure
2AU3 is a Single protein structure of sequence from Aquifex aeolicus with as ligand. Full crystallographic information is available from OCA.
Reference
Crosstalk between primase subunits can act to regulate primer synthesis in trans., Corn JE, Pease PJ, Hura GL, Berger JM, Mol Cell. 2005 Nov 11;20(3):391-401. PMID:16285921
Page seeded by OCA on Thu Feb 21 16:31:06 2008
