2auk
From Proteopedia
(New page: 200px<br /><applet load="2auk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2auk, resolution 2.3Å" /> '''Structure of E. coli ...) |
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| - | [[Image:2auk.gif|left|200px]]<br /><applet load="2auk" size=" | + | [[Image:2auk.gif|left|200px]]<br /><applet load="2auk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2auk, resolution 2.3Å" /> | caption="2auk, resolution 2.3Å" /> | ||
'''Structure of E. coli RNA polymerase beta' G/G' insert'''<br /> | '''Structure of E. coli RNA polymerase beta' G/G' insert'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The large beta and beta' subunits of the bacterial core RNA polymerase | + | The large beta and beta' subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in beta and beta' characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP beta' subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP beta' subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two beta' insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 2AUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 2AUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chlenov, M.]] | [[Category: Chlenov, M.]] | ||
| - | [[Category: Darst, S | + | [[Category: Darst, S A.]] |
[[Category: Masuda, S.]] | [[Category: Masuda, S.]] | ||
| - | [[Category: Murakami, K | + | [[Category: Murakami, K S.]] |
[[Category: Mustaev, A.]] | [[Category: Mustaev, A.]] | ||
[[Category: Nikiforov, V.]] | [[Category: Nikiforov, V.]] | ||
[[Category: sandwich-barrel hybrid motif]] | [[Category: sandwich-barrel hybrid motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:07 2008'' |
Revision as of 14:31, 21 February 2008
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Structure of E. coli RNA polymerase beta' G/G' insert
Overview
The large beta and beta' subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in beta and beta' characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP beta' subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP beta' subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two beta' insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions.
About this Structure
2AUK is a Single protein structure of sequence from Escherichia coli. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase beta' subunit., Chlenov M, Masuda S, Murakami KS, Nikiforov V, Darst SA, Mustaev A, J Mol Biol. 2005 Oct 14;353(1):138-54. PMID:16154587
Page seeded by OCA on Thu Feb 21 16:31:07 2008
