1mel
From Proteopedia
| Line 1: | Line 1: | ||
| - | {{Seed}} | ||
[[Image:1mel.png|left|200px]] | [[Image:1mel.png|left|200px]] | ||
| - | <!-- | ||
| - | The line below this paragraph, containing "STRUCTURE_1mel", creates the "Structure Box" on the page. | ||
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet) | ||
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| - | or leave the SCENE parameter empty for the default display. | ||
| - | --> | ||
{{STRUCTURE_1mel| PDB=1mel | SCENE= }} | {{STRUCTURE_1mel| PDB=1mel | SCENE= }} | ||
===CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME=== | ===CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME=== | ||
| - | |||
| - | <!-- | ||
| - | The line below this paragraph, {{ABSTRACT_PUBMED_8784355}}, adds the Publication Abstract to the page | ||
| - | (as it appears on PubMed at http://www.pubmed.gov), where 8784355 is the PubMed ID number. | ||
| - | --> | ||
{{ABSTRACT_PUBMED_8784355}} | {{ABSTRACT_PUBMED_8784355}} | ||
==About this Structure== | ==About this Structure== | ||
| - | + | [[1mel]] is a 4 chain structure of [[Hen Egg-White (HEW) Lysozyme]] with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. The April 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Nanobodies'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_4 10.2210/rcsb_pdb/mom_2011_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEL OCA]. | |
| + | |||
| + | ==See Also== | ||
| + | *[[Hen Egg-White (HEW) Lysozyme|Hen Egg-White (HEW) Lysozyme]] | ||
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:008784355</ref><ref group="xtra">PMID:010737939</ref><references group="xtra"/> |
[[Category: Camelus dromedarius]] | [[Category: Camelus dromedarius]] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
| + | [[Category: Nanobodies]] | ||
| + | [[Category: RCSB PDB Molecule of the Month]] | ||
[[Category: Desmyter, A.]] | [[Category: Desmyter, A.]] | ||
[[Category: Gharoudi, M Arbabi.]] | [[Category: Gharoudi, M Arbabi.]] | ||
| Line 36: | Line 29: | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: Camel single-domain anti-lysozyme]] | [[Category: Camel single-domain anti-lysozyme]] | ||
| - | |||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:34:41 2009'' | ||
Revision as of 09:32, 26 July 2012
| |||||||||
| 1mel, resolution 2.50Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | ANTIBODY VH FRAGMENT CAB-LYS3 (Camelus dromedarius), ANTIBODY VH FRAGMENT CAB-LYS3 (Gallus gallus) | ||||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME
The Camelidae is the only taxonomic family known to possess functional heavy-chain antibodies, lacking light chains. We report here the 2.5 A resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major backbone rearrangements in the VH framework. However, the architecture of the region of VH that interacts with a VL in a conventional FV is different from any previously seen. Moreover, the CDR1 region, although in sequence homologous to human CDR1, deviates fundamentally from the canonical structure. Additionally, one half of the CDR3 contacts the VH region which in conventional immunoglobulins interacts with a VL whereas the other half protrudes from the antigen binding site and penetrates deeply into the active site of lysozyme.
Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme., Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L, Nat Struct Biol. 1996 Sep;3(9):803-11. PMID:8784355
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1mel is a 4 chain structure of Hen Egg-White (HEW) Lysozyme with sequence from Camelus dromedarius and Gallus gallus. The April 2011 RCSB PDB Molecule of the Month feature on Nanobodies by David Goodsell is 10.2210/rcsb_pdb/mom_2011_4. Full crystallographic information is available from OCA.
See Also
Reference
- Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L. Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Nat Struct Biol. 1996 Sep;3(9):803-11. PMID:8784355
- Ritchie DW, Kemp GJ. Protein docking using spherical polar Fourier correlations. Proteins. 2000 May 1;39(2):178-94. PMID:10737939
