2b3d
From Proteopedia
(New page: 200px<br /><applet load="2b3d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b3d, resolution 2.1Å" /> '''Crystal structure of ...) |
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| - | [[Image:2b3d.gif|left|200px]]<br /><applet load="2b3d" size=" | + | [[Image:2b3d.gif|left|200px]]<br /><applet load="2b3d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2b3d, resolution 2.1Å" /> | caption="2b3d, resolution 2.1Å" /> | ||
'''Crystal structure of Modulator of Drug activity B in complex with flavin adenine dinucleotide'''<br /> | '''Crystal structure of Modulator of Drug activity B in complex with flavin adenine dinucleotide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Modulator of drug activity B (MdaB) is a putative member of the | + | Modulator of drug activity B (MdaB) is a putative member of the DT-diaphorase family of NAD(P)H:oxidoreductases that afford cellular protection against quinonoid compounds. While there have been extensive investigations of mammalian homologues, putative prokaryotic members of this enzyme family have received little attention. The three-dimensional crystal structure of apo-MdaB reported herein exhibits significant structural similarity to a number of flavoproteins, including the mammalian DT-diaphorases. We have shown by mass spectrometry that the endogenously associated cofactor is flavin adenine dinucleotide and we present here the structure of MdaB in complex with this compound. Growth of Escherichia coli carrying null mutations in the genes encoding MdaB or quinol monooxygenase, the gene for which shares the mdaB promoter, were not affected by the presence of menadione. However, over-expression of recombinant quinol monooxygenase conferred a state of resistance against both tetracycline and adriamycin. This work suggests that the redox cycle formed by these proteins protects E. coli from the toxic effects of polyketide compounds rather than the oxidative stress of menadione alone. |
==About this Structure== | ==About this Structure== | ||
| - | 2B3D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2B3D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Adams, M | + | [[Category: Adams, M A.]] |
[[Category: Jia, Z.]] | [[Category: Jia, Z.]] | ||
[[Category: FAD]] | [[Category: FAD]] | ||
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[[Category: nad(p)h:oxidoreductase; dt-diaphorase; menadione reductase; quinone]] | [[Category: nad(p)h:oxidoreductase; dt-diaphorase; menadione reductase; quinone]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:42 2008'' |
Revision as of 14:33, 21 February 2008
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Crystal structure of Modulator of Drug activity B in complex with flavin adenine dinucleotide
Overview
Modulator of drug activity B (MdaB) is a putative member of the DT-diaphorase family of NAD(P)H:oxidoreductases that afford cellular protection against quinonoid compounds. While there have been extensive investigations of mammalian homologues, putative prokaryotic members of this enzyme family have received little attention. The three-dimensional crystal structure of apo-MdaB reported herein exhibits significant structural similarity to a number of flavoproteins, including the mammalian DT-diaphorases. We have shown by mass spectrometry that the endogenously associated cofactor is flavin adenine dinucleotide and we present here the structure of MdaB in complex with this compound. Growth of Escherichia coli carrying null mutations in the genes encoding MdaB or quinol monooxygenase, the gene for which shares the mdaB promoter, were not affected by the presence of menadione. However, over-expression of recombinant quinol monooxygenase conferred a state of resistance against both tetracycline and adriamycin. This work suggests that the redox cycle formed by these proteins protects E. coli from the toxic effects of polyketide compounds rather than the oxidative stress of menadione alone.
About this Structure
2B3D is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Modulator of drug activity B from Escherichia coli: crystal structure of a prokaryotic homologue of DT-diaphorase., Adams MA, Jia Z, J Mol Biol. 2006 Jun 2;359(2):455-65. Epub 2006 Apr 7. PMID:16630630
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