2b5l
From Proteopedia
(New page: 200px<br /><applet load="2b5l" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b5l, resolution 2.85Å" /> '''Crystal Structure of...) |
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| - | [[Image:2b5l.gif|left|200px]]<br /><applet load="2b5l" size=" | + | [[Image:2b5l.gif|left|200px]]<br /><applet load="2b5l" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2b5l, resolution 2.85Å" /> | caption="2b5l, resolution 2.85Å" /> | ||
'''Crystal Structure of DDB1 In Complex with Simian Virus 5 V Protein'''<br /> | '''Crystal Structure of DDB1 In Complex with Simian Virus 5 V Protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The DDB1-Cul4A ubiquitin ligase complex promotes protein ubiquitination in | + | The DDB1-Cul4A ubiquitin ligase complex promotes protein ubiquitination in diverse cellular functions and is reprogrammed by the V proteins of paramyxoviruses to degrade STATs and block interferon signaling. Here we report the crystal structures of DDB1 alone and in complex with the simian virus 5 V protein. The DDB1 structure reveals an intertwined three-propeller cluster, which contains two tightly coupled beta propellers with a large pocket in between and a third beta propeller flexibly attached on the side. The rigid double-propeller fold of DDB1 is targeted by the viral V protein, which inserts an entire helix into the double-propeller pocket, whereas the third propeller domain docks DDB1 to the N terminus of the Cul4A scaffold. Together, these results not only provide structural insights into how the virus hijacks the DDB1-Cul4A ubiquitin ligase but also establish a structural framework for understanding the multiple functions of DDB1 in the uniquely assembled cullin-RING E3 machinery. |
==About this Structure== | ==About this Structure== | ||
| - | 2B5L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2B5L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
[[Category: Chen, X.]] | [[Category: Chen, X.]] | ||
| - | [[Category: Garbutt, K | + | [[Category: Garbutt, K C.]] |
[[Category: Li, T.]] | [[Category: Li, T.]] | ||
[[Category: Zheng, N.]] | [[Category: Zheng, N.]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:24 2008'' |
Revision as of 14:34, 21 February 2008
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Crystal Structure of DDB1 In Complex with Simian Virus 5 V Protein
Overview
The DDB1-Cul4A ubiquitin ligase complex promotes protein ubiquitination in diverse cellular functions and is reprogrammed by the V proteins of paramyxoviruses to degrade STATs and block interferon signaling. Here we report the crystal structures of DDB1 alone and in complex with the simian virus 5 V protein. The DDB1 structure reveals an intertwined three-propeller cluster, which contains two tightly coupled beta propellers with a large pocket in between and a third beta propeller flexibly attached on the side. The rigid double-propeller fold of DDB1 is targeted by the viral V protein, which inserts an entire helix into the double-propeller pocket, whereas the third propeller domain docks DDB1 to the N terminus of the Cul4A scaffold. Together, these results not only provide structural insights into how the virus hijacks the DDB1-Cul4A ubiquitin ligase but also establish a structural framework for understanding the multiple functions of DDB1 in the uniquely assembled cullin-RING E3 machinery.
About this Structure
2B5L is a Protein complex structure of sequences from Homo sapiens and Simian virus 40 with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase., Li T, Chen X, Garbutt KC, Zhou P, Zheng N, Cell. 2006 Jan 13;124(1):105-17. PMID:16413485
Page seeded by OCA on Thu Feb 21 16:34:24 2008
Categories: Homo sapiens | Protein complex | Simian virus 40 | Chen, X. | Garbutt, K C. | Li, T. | Zheng, N. | Zhou, P. | ZN | Beta propeller | Ddb1 | Propeller cluster | Sv5-v | Zinc finger
