2b9o
From Proteopedia
(New page: 200px<br /><applet load="2b9o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b9o, resolution 6.46Å" /> '''30S ribosomal subuni...) |
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| - | [[Image:2b9o.gif|left|200px]]<br /><applet load="2b9o" size=" | + | [[Image:2b9o.gif|left|200px]]<br /><applet load="2b9o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2b9o, resolution 6.46Å" /> | caption="2b9o, resolution 6.46Å" /> | ||
'''30S ribosomal subunit, tRNAs and mRNA from a crystal structure of the whole ribosomal complex with a stop codon in the A-site. This file contains the 30S subunit, tRNAs and mRNA from a crystal structure of the whole ribosomal complex with a stop codon in the A-site and is described in remark 400.'''<br /> | '''30S ribosomal subunit, tRNAs and mRNA from a crystal structure of the whole ribosomal complex with a stop codon in the A-site. This file contains the 30S subunit, tRNAs and mRNA from a crystal structure of the whole ribosomal complex with a stop codon in the A-site and is described in remark 400.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During protein synthesis, translational release factors catalyze the | + | During protein synthesis, translational release factors catalyze the release of the polypeptide chain when a stop codon on the mRNA reaches the A site of the ribosome. The detailed mechanism of this process is currently unknown. We present here the crystal structures of the ribosome from Thermus thermophilus with RF1 and RF2 bound to their cognate stop codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The structures reveal details of interactions of the factors with the ribosome and mRNA, including elements previously implicated in decoding and peptide release. They also shed light on conformational changes both in the factors and in the ribosome during termination. Differences seen in the interaction of RF1 and RF2 with the L11 region of the ribosome allow us to rationalize previous biochemical data. Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway. |
==About this Structure== | ==About this Structure== | ||
| - | 2B9O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] and [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http:// | + | 2B9O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] and [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Thermus thermophilus hb27]] | [[Category: Thermus thermophilus hb27]] | ||
| - | [[Category: Brodersen, D | + | [[Category: Brodersen, D E.]] |
| - | [[Category: Dunham, C | + | [[Category: Dunham, C M.]] |
| - | [[Category: IV, F | + | [[Category: IV, F V.Murphy.]] |
| - | [[Category: Kelley, A | + | [[Category: Kelley, A C.]] |
[[Category: Petry, S.]] | [[Category: Petry, S.]] | ||
[[Category: Ramakrishnan, V.]] | [[Category: Ramakrishnan, V.]] | ||
[[Category: Selmer, M.]] | [[Category: Selmer, M.]] | ||
| - | [[Category: Tarry, M | + | [[Category: Tarry, M J.]] |
[[Category: ribosome]] | [[Category: ribosome]] | ||
[[Category: translation]] | [[Category: translation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:35:31 2008'' |
Revision as of 14:35, 21 February 2008
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30S ribosomal subunit, tRNAs and mRNA from a crystal structure of the whole ribosomal complex with a stop codon in the A-site. This file contains the 30S subunit, tRNAs and mRNA from a crystal structure of the whole ribosomal complex with a stop codon in the A-site and is described in remark 400.
Overview
During protein synthesis, translational release factors catalyze the release of the polypeptide chain when a stop codon on the mRNA reaches the A site of the ribosome. The detailed mechanism of this process is currently unknown. We present here the crystal structures of the ribosome from Thermus thermophilus with RF1 and RF2 bound to their cognate stop codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The structures reveal details of interactions of the factors with the ribosome and mRNA, including elements previously implicated in decoding and peptide release. They also shed light on conformational changes both in the factors and in the ribosome during termination. Differences seen in the interaction of RF1 and RF2 with the L11 region of the ribosome allow us to rationalize previous biochemical data. Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway.
About this Structure
2B9O is a Protein complex structure of sequences from Escherichia coli, Thermus thermophilus and Thermus thermophilus hb27. Full crystallographic information is available from OCA.
Reference
Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon., Petry S, Brodersen DE, Murphy FV 4th, Dunham CM, Selmer M, Tarry MJ, Kelley AC, Ramakrishnan V, Cell. 2005 Dec 29;123(7):1255-66. PMID:16377566
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