3bir
From Proteopedia
(New page: 200px<br /> <applet load="3bir" size="450" color="white" frame="true" align="right" spinBox="true" caption="3bir, resolution 1.80Å" /> '''DISECTING HISTIDINE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 3BIR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]] with CA and 2GP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3BIR OCA]]. | + | 3BIR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]] with CA and 2GP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3BIR OCA]]. |
==Reference== | ==Reference== | ||
Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position., De Vos S, Doumen J, Langhorst U, Steyaert J, J Mol Biol. 1998 Jan 30;275(4):651-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466938 9466938] | Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position., De Vos S, Doumen J, Langhorst U, Steyaert J, J Mol Biol. 1998 Jan 30;275(4):651-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466938 9466938] | ||
[[Category: Aspergillus oryzae]] | [[Category: Aspergillus oryzae]] | ||
| + | [[Category: Ribonuclease T(1)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Doumen, J.]] | [[Category: Doumen, J.]] | ||
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[[Category: ribonuclease t1]] | [[Category: ribonuclease t1]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:16:25 2007'' |
Revision as of 12:11, 30 October 2007
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DISECTING HISTIDINE INTERACTIONS IN RIBONUCLEASE T1 BY ASN AND GLN SUBSTITUTIONS
Overview
His92 of Ribonuclease T1 combines functional and structural features, involving both imidazole nitrogens. To evaluate the use of Asn and Gln, substitutions in dissecting the properties of histidines, we analysed the, consequences of the His92Gln and His92Asn substitutions on the enzyme's, structure, function, and conformational stability by protein engineering, and X-ray crystallographic methods. In the X-ray structures of wild-type, and His92Gln RNase T1 in complex with 2'-GMP the His92-N epsilon 2 and, Gln92-N epsilon 2 atoms are isosterically equivalent. Similarly, the, His92N delta 1H...OAsn99 hydrogen bond observed in wild-type is replaced, by an equivalent Asn92N delta 2H...OAsn99 in the His92Asn mutant, structure. Double mutant cycles at a single position were used to analyse, the ... [(full description)]
About this Structure
3BIR is a [Single protein] structure of sequence from [Aspergillus oryzae] with CA and 2GP as [ligands]. Active as [Ribonuclease T(1)], with EC number [3.1.27.3]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position., De Vos S, Doumen J, Langhorst U, Steyaert J, J Mol Biol. 1998 Jan 30;275(4):651-61. PMID:9466938
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