2ccz
From Proteopedia
(New page: 200px<br /><applet load="2ccz" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ccz, resolution 2.70Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:2ccz.gif|left|200px]]<br /><applet load="2ccz" size=" | + | [[Image:2ccz.gif|left|200px]]<br /><applet load="2ccz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ccz, resolution 2.70Å" /> | caption="2ccz, resolution 2.70Å" /> | ||
'''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA'''<br /> | '''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CCZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2CCZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ssdna]] | [[Category: ssdna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:57:14 2008'' |
Revision as of 12:57, 23 January 2008
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CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA
Overview
PriB is a primosomal protein required for replication restart in, Escherichia coli. PriB stimulates PriA helicase activity via interaction, with single-stranded DNA (ssDNA), but the molecular details of this, interaction remain unclear. Here, we report the crystal structure of PriB, complexed with a 15 bases oligonucleotide (dT15) at 2.7 A resolution. PriB, shares structural similarity with the E.coli ssDNA-binding protein, (EcoSSB). However, the structure of the PriB-dT15 complex reveals that, PriB binds ssDNA differently. Results from filter-binding assays show that, PriB-ssDNA interaction is salt-sensitive and cooperative. Mutational, analysis suggests that the loop L45 plays an important role in ssDNA, binding. Based on the crystal structure and biochemical analyses, we, propose a cooperative mechanism for the binding of PriB to ssDNA and a, model for the assembly of the PriA-PriB-ssDNA complex. This report, presents the first structure of a replication restart primosomal protein, complexed with DNA, and a novel model that explains the interactions, between a dimeric oligonucleotide-binding-fold protein and ssDNA.
About this Structure
2CCZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode., Huang CY, Hsu CH, Sun YJ, Wu HN, Hsiao CD, Nucleic Acids Res. 2006;34(14):3878-86. Epub 2006 Aug 9. PMID:16899446
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