2cge
From Proteopedia
(New page: 200px<br /><applet load="2cge" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cge, resolution 3.00Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:2cge.gif|left|200px]]<br /><applet load="2cge" size=" | + | [[Image:2cge.gif|left|200px]]<br /><applet load="2cge" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cge, resolution 3.00Å" /> | caption="2cge, resolution 3.00Å" /> | ||
'''CRYSTAL STRUCTURE OF AN HSP90-SBA1 CLOSED CHAPERONE COMPLEX'''<br /> | '''CRYSTAL STRUCTURE OF AN HSP90-SBA1 CLOSED CHAPERONE COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Hsp90 (heat shock protein of 90 kDa) is a ubiquitous molecular chaperone | + | Hsp90 (heat shock protein of 90 kDa) is a ubiquitous molecular chaperone responsible for the assembly and regulation of many eukaryotic signalling systems and is an emerging target for rational chemotherapy of many cancers. Although the structures of isolated domains of Hsp90 have been determined, the arrangement and ATP-dependent dynamics of these in the full Hsp90 dimer have been elusive and contentious. Here we present the crystal structure of full-length yeast Hsp90 in complex with an ATP analogue and the co-chaperone p23/Sba1. The structure reveals the complex architecture of the 'closed' state of the Hsp90 chaperone, the extensive interactions between domains and between protein chains, the detailed conformational changes in the amino-terminal domain that accompany ATP binding, and the structural basis for stabilization of the closed state by p23/Sba1. Contrary to expectations, the closed Hsp90 would not enclose its client proteins but provides a bipartite binding surface whose formation and disruption are coupled to the chaperone ATPase cycle. |
==About this Structure== | ==About this Structure== | ||
| - | 2CGE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2CGE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ali, M | + | [[Category: Ali, M M.U.]] |
| - | [[Category: Pearl, L | + | [[Category: Pearl, L H.]] |
[[Category: Prodromou, C.]] | [[Category: Prodromou, C.]] | ||
| - | [[Category: Roe, S | + | [[Category: Roe, S M.]] |
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: chaperone]] | [[Category: chaperone]] | ||
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[[Category: nucleotide-binding]] | [[Category: nucleotide-binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:48:24 2008'' |
Revision as of 14:48, 21 February 2008
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CRYSTAL STRUCTURE OF AN HSP90-SBA1 CLOSED CHAPERONE COMPLEX
Overview
Hsp90 (heat shock protein of 90 kDa) is a ubiquitous molecular chaperone responsible for the assembly and regulation of many eukaryotic signalling systems and is an emerging target for rational chemotherapy of many cancers. Although the structures of isolated domains of Hsp90 have been determined, the arrangement and ATP-dependent dynamics of these in the full Hsp90 dimer have been elusive and contentious. Here we present the crystal structure of full-length yeast Hsp90 in complex with an ATP analogue and the co-chaperone p23/Sba1. The structure reveals the complex architecture of the 'closed' state of the Hsp90 chaperone, the extensive interactions between domains and between protein chains, the detailed conformational changes in the amino-terminal domain that accompany ATP binding, and the structural basis for stabilization of the closed state by p23/Sba1. Contrary to expectations, the closed Hsp90 would not enclose its client proteins but provides a bipartite binding surface whose formation and disruption are coupled to the chaperone ATPase cycle.
About this Structure
2CGE is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of an Hsp90-nucleotide-p23/Sba1 closed chaperone complex., Ali MM, Roe SM, Vaughan CK, Meyer P, Panaretou B, Piper PW, Prodromou C, Pearl LH, Nature. 2006 Apr 20;440(7087):1013-7. PMID:16625188
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