2cua
From Proteopedia
(New page: 200px<br /><applet load="2cua" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cua, resolution 1.60Å" /> '''THE CUA DOMAIN OF CY...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2cua.jpg|left|200px]]<br /><applet load="2cua" size=" | + | [[Image:2cua.jpg|left|200px]]<br /><applet load="2cua" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cua, resolution 1.60Å" /> | caption="2cua, resolution 1.60Å" /> | ||
'''THE CUA DOMAIN OF CYTOCHROME BA3 FROM THERMUS THERMOPHILUS'''<br /> | '''THE CUA DOMAIN OF CYTOCHROME BA3 FROM THERMUS THERMOPHILUS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the CuA-containing, extracellular domain of Thermus | + | The structure of the CuA-containing, extracellular domain of Thermus thermophilus ba3-type cytochrome c oxidase has been determined to 1.6 A resolution using multiple X-ray wavelength anomalous dispersion (MAD). The Cu2S2 cluster forms a planar rhombus with a copper-copper distance of 2.51 +/- 0.03 A. X-ray absorption fine-structure (EXAFS) studies show that this distance is unchanged by crystallization. The CuA center is asymmetrical; one copper is tetrahedrally coordinated to two bridging cysteine thiolates, one histidine nitrogen and one methionine sulfur, while the other is trigonally coordinated by the two cysteine thiolates and a histidine nitrogen. Combined sequence-structure alignment of amino acid sequences reveals conserved interactions between cytochrome c oxidase subunits I and II. |
==About this Structure== | ==About this Structure== | ||
| - | 2CUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN and CUA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c_oxidase Cytochrome-c oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1] Full crystallographic information is available from [http:// | + | 2CUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CUA:'>CUA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c_oxidase Cytochrome-c oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CUA OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Bellamy, H.]] | [[Category: Bellamy, H.]] | ||
| - | [[Category: Blackburn, N | + | [[Category: Blackburn, N J.]] |
| - | [[Category: Fee, J | + | [[Category: Fee, J A.]] |
| - | [[Category: Mcree, D | + | [[Category: Mcree, D E.]] |
[[Category: Sanders, D.]] | [[Category: Sanders, D.]] | ||
| - | [[Category: Stura, E | + | [[Category: Stura, E A.]] |
| - | [[Category: Williams, P | + | [[Category: Williams, P A.]] |
[[Category: CUA]] | [[Category: CUA]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: cua center]] | [[Category: cua center]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:52:28 2008'' |
Revision as of 14:52, 21 February 2008
|
THE CUA DOMAIN OF CYTOCHROME BA3 FROM THERMUS THERMOPHILUS
Overview
The structure of the CuA-containing, extracellular domain of Thermus thermophilus ba3-type cytochrome c oxidase has been determined to 1.6 A resolution using multiple X-ray wavelength anomalous dispersion (MAD). The Cu2S2 cluster forms a planar rhombus with a copper-copper distance of 2.51 +/- 0.03 A. X-ray absorption fine-structure (EXAFS) studies show that this distance is unchanged by crystallization. The CuA center is asymmetrical; one copper is tetrahedrally coordinated to two bridging cysteine thiolates, one histidine nitrogen and one methionine sulfur, while the other is trigonally coordinated by the two cysteine thiolates and a histidine nitrogen. Combined sequence-structure alignment of amino acid sequences reveals conserved interactions between cytochrome c oxidase subunits I and II.
About this Structure
2CUA is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Active as Cytochrome-c oxidase, with EC number 1.9.3.1 Full crystallographic information is available from OCA.
Reference
The CuA domain of Thermus thermophilus ba3-type cytochrome c oxidase at 1.6 A resolution., Williams PA, Blackburn NJ, Sanders D, Bellamy H, Stura EA, Fee JA, McRee DE, Nat Struct Biol. 1999 Jun;6(6):509-16. PMID:10360350
Page seeded by OCA on Thu Feb 21 16:52:28 2008
