2cv2
From Proteopedia
(New page: 200px<br /><applet load="2cv2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cv2, resolution 2.69Å" /> '''Glutamyl-tRNA synthe...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2cv2.gif|left|200px]]<br /><applet load="2cv2" size=" | + | [[Image:2cv2.gif|left|200px]]<br /><applet load="2cv2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cv2, resolution 2.69Å" /> | caption="2cv2, resolution 2.69Å" /> | ||
'''Glutamyl-tRNA synthetase from Thermus thermophilus in complex with tRNA(Glu) and an enzyme inhibitor, Glu-AMS'''<br /> | '''Glutamyl-tRNA synthetase from Thermus thermophilus in complex with tRNA(Glu) and an enzyme inhibitor, Glu-AMS'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2CV2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG, CL and GSU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamate--tRNA_ligase Glutamate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17] Full crystallographic information is available from [http:// | + | 2CV2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=GSU:'>GSU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamate--tRNA_ligase Glutamate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CV2 OCA]. |
==Reference== | ==Reference== | ||
| Line 28: | Line 28: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:48:49 2008'' |
Revision as of 11:48, 23 January 2008
|
Glutamyl-tRNA synthetase from Thermus thermophilus in complex with tRNA(Glu) and an enzyme inhibitor, Glu-AMS
Overview
Glutamyl-tRNA synthetase (GluRS) is one of the aminoacyl-tRNA synthetases, that require the cognate tRNA for specific amino acid recognition and, activation. We analyzed the role of tRNA in amino acid recognition by, crystallography. In the GluRS*tRNA(Glu)*Glu structure, GluRS and tRNA(Glu), collaborate to form a highly complementary L-glutamate-binding site. This, collaborative site is functional, as it is formed in the same manner in, pretransition-state mimic, GluRS*tRNA(Glu)*ATP*Eol (a glutamate analog), and posttransition-state mimic, GluRS*tRNA(Glu)*ESA (a glutamyl-adenylate, analog) structures. In contrast, in the GluRS*Glu structure, only GluRS, forms the amino acid-binding site, which is defective and accounts for the, binding of incorrect amino acids, such as D-glutamate and L-glutamine., Therefore, tRNA(Glu) is essential for formation of the completely, functional binding site for L-glutamate. These structures, together with, our previously described structures, reveal that tRNA plays a crucial role, in accurate positioning of both L-glutamate and ATP, thus driving the, amino acid activation.
About this Structure
2CV2 is a Single protein structure of sequence from Thermus thermophilus with , and as ligands. Active as Glutamate--tRNA ligase, with EC number 6.1.1.17 Full crystallographic information is available from OCA.
Reference
Structural bases of transfer RNA-dependent amino acid recognition and activation by glutamyl-tRNA synthetase., Sekine S, Shichiri M, Bernier S, Chenevert R, Lapointe J, Yokoyama S, Structure. 2006 Dec;14(12):1791-9. PMID:17161369
Page seeded by OCA on Wed Jan 23 13:48:49 2008
Categories: Glutamate--tRNA ligase | Single protein | Thermus thermophilus | RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative. | Sekine, S. | Yokoyama, S. | CL | GSU | MG | Ligase | National project on protein structural and functional analyses | Nppsfa | Riken structural genomics/proteomics initiative | Rna | Rsgi | Structural genomics
