2czu
From Proteopedia
(New page: 200px<br /><applet load="2czu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2czu, resolution 2.10Å" /> '''lipocalin-type prost...) |
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| - | [[Image:2czu.gif|left|200px]]<br /><applet load="2czu" size=" | + | [[Image:2czu.gif|left|200px]]<br /><applet load="2czu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2czu, resolution 2.10Å" /> | caption="2czu, resolution 2.10Å" /> | ||
'''lipocalin-type prostaglandin D synthase'''<br /> | '''lipocalin-type prostaglandin D synthase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Lipocalin-type prostaglandin D synthase is the key enzyme for the | + | Lipocalin-type prostaglandin D synthase is the key enzyme for the production of prostaglandin D(2), a potent endogenous somnogen, in the brain. We cloned, produced, and crystallized the native enzyme and selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the hanging drop vapor-diffusion method with both malonate and citrate as precipitants. The native crystals obtained with malonate belong to orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with citrate belong to orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond 2.1 A resolution. |
==About this Structure== | ==About this Structure== | ||
| - | 2CZU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] Full crystallographic information is available from [http:// | + | 2CZU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZU OCA]. |
==Reference== | ==Reference== | ||
| - | Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem | + | Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12761195 12761195] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Prostaglandin-D synthase]] | [[Category: Prostaglandin-D synthase]] | ||
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[[Category: Kumasaka, T.]] | [[Category: Kumasaka, T.]] | ||
[[Category: Miyano, M.]] | [[Category: Miyano, M.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Urade, Y.]] | [[Category: Urade, Y.]] | ||
[[Category: Yamamoto, M.]] | [[Category: Yamamoto, M.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:53:59 2008'' |
Revision as of 14:54, 21 February 2008
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lipocalin-type prostaglandin D synthase
Overview
Lipocalin-type prostaglandin D synthase is the key enzyme for the production of prostaglandin D(2), a potent endogenous somnogen, in the brain. We cloned, produced, and crystallized the native enzyme and selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the hanging drop vapor-diffusion method with both malonate and citrate as precipitants. The native crystals obtained with malonate belong to orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with citrate belong to orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond 2.1 A resolution.
About this Structure
2CZU is a Single protein structure of sequence from Mus musculus. Active as Prostaglandin-D synthase, with EC number 5.3.99.2 Full crystallographic information is available from OCA.
Reference
Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:12761195
Page seeded by OCA on Thu Feb 21 16:53:59 2008
Categories: Mus musculus | Prostaglandin-D synthase | Single protein | Ago, H. | Aritake, K. | Hayaishi, O. | Inoue, T. | Irikura, D. | Kumasaka, T. | Miyano, M. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Urade, Y. | Yamamoto, M. | Lipocalin | Lpgds p212121 native | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics
