2d00
From Proteopedia
(New page: 200px<br /><applet load="2d00" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d00, resolution 2.2Å" /> '''Subunit F of V-type A...) |
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| - | [[Image:2d00.gif|left|200px]]<br /><applet load="2d00" size=" | + | [[Image:2d00.gif|left|200px]]<br /><applet load="2d00" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2d00, resolution 2.2Å" /> | caption="2d00, resolution 2.2Å" /> | ||
'''Subunit F of V-type ATPase/synthase'''<br /> | '''Subunit F of V-type ATPase/synthase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of subunit F of vacuole-type ATPase/synthase | + | The crystal structure of subunit F of vacuole-type ATPase/synthase (prokaryotic V-ATPase) was determined to of 2.2 A resolution. The subunit reveals unexpected structural similarity to the response regulator proteins that include the Escherichia coli chemotaxis response regulator CheY. The structure was successfully placed into the low-resolution EM structure of the prokaryotic holo-V-ATPase at a location indicated by the results of crosslinking experiments. The crystal structure, together with the single-molecule analysis using fluorescence resonance energy transfer, showed that the subunit F exhibits two conformations, a 'retracted' form in the absence and an 'extended' form in the presence of ATP. Our results postulated that the subunit F is a regulatory subunit in the V-ATPase. |
==About this Structure== | ==About this Structure== | ||
| - | 2D00 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Full crystallographic information is available from [http:// | + | 2D00 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D00 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
| - | [[Category: Bernal, R | + | [[Category: Bernal, R A.]] |
| - | [[Category: Carpenter, E | + | [[Category: Carpenter, E P.]] |
[[Category: Iino, R.]] | [[Category: Iino, R.]] | ||
[[Category: Ikeda, C.]] | [[Category: Ikeda, C.]] | ||
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[[Category: v-atpase]] | [[Category: v-atpase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:54:01 2008'' |
Revision as of 14:54, 21 February 2008
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Subunit F of V-type ATPase/synthase
Overview
The crystal structure of subunit F of vacuole-type ATPase/synthase (prokaryotic V-ATPase) was determined to of 2.2 A resolution. The subunit reveals unexpected structural similarity to the response regulator proteins that include the Escherichia coli chemotaxis response regulator CheY. The structure was successfully placed into the low-resolution EM structure of the prokaryotic holo-V-ATPase at a location indicated by the results of crosslinking experiments. The crystal structure, together with the single-molecule analysis using fluorescence resonance energy transfer, showed that the subunit F exhibits two conformations, a 'retracted' form in the absence and an 'extended' form in the presence of ATP. Our results postulated that the subunit F is a regulatory subunit in the V-ATPase.
About this Structure
2D00 is a Single protein structure of sequence from Thermus thermophilus with as ligand. Active as H(+)-transporting two-sector ATPase, with EC number 3.6.3.14 Full crystallographic information is available from OCA.
Reference
Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus., Makyio H, Iino R, Ikeda C, Imamura H, Tamakoshi M, Iwata M, Stock D, Bernal RA, Carpenter EP, Yoshida M, Yokoyama K, Iwata S, EMBO J. 2005 Nov 16;24(22):3974-83. Epub 2005 Nov 10. PMID:16281059
Page seeded by OCA on Thu Feb 21 16:54:01 2008
Categories: H(+)-transporting two-sector ATPase | Single protein | Thermus thermophilus | Bernal, R A. | Carpenter, E P. | Iino, R. | Ikeda, C. | Imamura, H. | Iwata, M. | Iwata, S. | Makyio, H. | Stock, D. | Tamakoshi, M. | Yokoyama, K. | Yoshida, M. | CA | Chey | Fret | Subunit f | V-atpase
