2d3d
From Proteopedia
(New page: 200px<br /><applet load="2d3d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d3d, resolution 1.60Å" /> '''crystal structure of...) |
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| - | [[Image:2d3d.gif|left|200px]]<br /><applet load="2d3d" size=" | + | [[Image:2d3d.gif|left|200px]]<br /><applet load="2d3d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2d3d, resolution 1.60Å" /> | caption="2d3d, resolution 1.60Å" /> | ||
'''crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1'''<br /> | '''crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The SAM domain of the Saccharomyces cerevisiae post-transcriptional | + | The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1 has a high affinity towards RNA hairpins containing a CUGGC pentaloop. We present the 1.6 Angstroms X-ray crystal structure of the Vts1 SAM domain in its unliganded state, and the NMR solution structure of this domain in its RNA-bound state. Both structures reveal a canonical five helix SAM domain flanked by additional secondary structural elements at the N and C termini. The two structures are essentially identical, implying that no major structural rearrangements occur upon RNA binding. Amide chemical shift changes map the RNA-binding site to a shallow, basic patch at the junction of helix alpha5 and the loop connecting helices alpha1 and alpha2. |
==About this Structure== | ==About this Structure== | ||
| - | 2D3D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2D3D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amborski, A | + | [[Category: Amborski, A N.]] |
[[Category: Aviv, T.]] | [[Category: Aviv, T.]] | ||
| - | [[Category: Donaldson, L | + | [[Category: Donaldson, L W.]] |
| - | [[Category: Johnson, P | + | [[Category: Johnson, P E.]] |
| - | [[Category: Kwan, J | + | [[Category: Kwan, J J.]] |
[[Category: Sicheri, F.]] | [[Category: Sicheri, F.]] | ||
| - | [[Category: Zhao, X | + | [[Category: Zhao, X S.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: rna binding]] | [[Category: rna binding]] | ||
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[[Category: sre hairpin binding]] | [[Category: sre hairpin binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:01 2008'' |
Revision as of 14:55, 21 February 2008
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crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1
Overview
The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1 has a high affinity towards RNA hairpins containing a CUGGC pentaloop. We present the 1.6 Angstroms X-ray crystal structure of the Vts1 SAM domain in its unliganded state, and the NMR solution structure of this domain in its RNA-bound state. Both structures reveal a canonical five helix SAM domain flanked by additional secondary structural elements at the N and C termini. The two structures are essentially identical, implying that no major structural rearrangements occur upon RNA binding. Amide chemical shift changes map the RNA-binding site to a shallow, basic patch at the junction of helix alpha5 and the loop connecting helices alpha1 and alpha2.
About this Structure
2D3D is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
The NMR and X-ray structures of the Saccharomyces cerevisiae Vts1 SAM domain define a surface for the recognition of RNA hairpins., Aviv T, Amborski AN, Zhao XS, Kwan JJ, Johnson PE, Sicheri F, Donaldson LW, J Mol Biol. 2006 Feb 17;356(2):274-9. Epub 2005 Dec 7. PMID:16375924
Page seeded by OCA on Thu Feb 21 16:55:01 2008
