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2d6b
From Proteopedia
(New page: 200px<br /><applet load="2d6b" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d6b, resolution 1.25Å" /> '''Novel Bromate Specie...) |
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| - | [[Image:2d6b.gif|left|200px]]<br /><applet load="2d6b" size=" | + | [[Image:2d6b.gif|left|200px]]<br /><applet load="2d6b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2d6b, resolution 1.25Å" /> | caption="2d6b, resolution 1.25Å" /> | ||
'''Novel Bromate Species trapped within a Protein Crystal'''<br /> | '''Novel Bromate Species trapped within a Protein Crystal'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Only a few protein-oxoanion crystal complexes have been described to date. | + | Only a few protein-oxoanion crystal complexes have been described to date. Here, the structure of a protein soaked in a bromate solution has been determined to a resolution of 1.25 A and refined to final overall R/R(free) values of 18.04/21.3 (isotropic) and 11.25/14.67 (anisotropic). In contrast to the single-model approach, refinement of an ensemble of ten models enabled us to determine variances and statistically evaluate bond-length distances and angles in the oxoanions. In total, nine bromate positions, including two BrO(3)(-) x HBrO(3) dimer species, have been identified on the basis of the anomalous signal of the Br atoms. For all bromate ions, the main-chain amide atoms of the protein were identified as the dominant binding positions, a useful property in any experimental phase-determination experiment. |
==About this Structure== | ==About this Structure== | ||
| - | 2D6B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CL, NA and 202 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 2D6B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=202:'>202</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D6B OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Mesters, J | + | [[Category: Mesters, J R.]] |
[[Category: Ondracek, J.]] | [[Category: Ondracek, J.]] | ||
[[Category: 202]] | [[Category: 202]] | ||
| Line 21: | Line 21: | ||
[[Category: lysozyme; bromate]] | [[Category: lysozyme; bromate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:53 2008'' |
Revision as of 14:55, 21 February 2008
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Novel Bromate Species trapped within a Protein Crystal
Overview
Only a few protein-oxoanion crystal complexes have been described to date. Here, the structure of a protein soaked in a bromate solution has been determined to a resolution of 1.25 A and refined to final overall R/R(free) values of 18.04/21.3 (isotropic) and 11.25/14.67 (anisotropic). In contrast to the single-model approach, refinement of an ensemble of ten models enabled us to determine variances and statistically evaluate bond-length distances and angles in the oxoanions. In total, nine bromate positions, including two BrO(3)(-) x HBrO(3) dimer species, have been identified on the basis of the anomalous signal of the Br atoms. For all bromate ions, the main-chain amide atoms of the protein were identified as the dominant binding positions, a useful property in any experimental phase-determination experiment.
About this Structure
2D6B is a Single protein structure of sequence from Gallus gallus with , and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
An ensemble of crystallographic models enables the description of novel bromate-oxoanion species trapped within a protein crystal., Ondracek J, Mesters JR, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):996-1001. Epub 2006, Aug 19. PMID:16929100
Page seeded by OCA on Thu Feb 21 16:55:53 2008
Categories: Gallus gallus | Lysozyme | Single protein | Mesters, J R. | Ondracek, J. | 202 | CL | NA | Lysozyme; bromate
