2de9
From Proteopedia
(New page: 200px<br /><applet load="2de9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2de9, resolution 1.3Å" /> '''Crystal structure of ...) |
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| - | [[Image:2de9.gif|left|200px]]<br /><applet load="2de9" size=" | + | [[Image:2de9.gif|left|200px]]<br /><applet load="2de9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2de9, resolution 1.3Å" /> | caption="2de9, resolution 1.3Å" /> | ||
'''Crystal structure of porcine pancreatic elastase complexed with Tris after soaking a tris-free solution'''<br /> | '''Crystal structure of porcine pancreatic elastase complexed with Tris after soaking a tris-free solution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Porcine pancreatic elastase (PPE) was crystallized under new sulfate-free | + | Porcine pancreatic elastase (PPE) was crystallized under new sulfate-free conditions containing 0.3 M NaCl and 50 mM tris(hydroxymethyl)aminomethane-HCl at pH 7.0. The crystal structure determined at 1.5 angstroms resolution had a unique conformation in four regions which contained loop portions. A chloride ion was bound near the catalytic triad instead of the sulfate ion in PDB entry 1qnj, a typical PPE crystal structure. However, the chloride ion did not affect the configuration of the catalytic triad. A tris(hydroxymethyl)aminomethane (Tris) molecule was bound to the S4 and S5 subsites in place of the adjacent molecule in the 1qnj crystal and played a significant role in the structural change of the region. The distortion in this region may subsequently have induced conformational changes in the other three regions. The fact that Tris and these four regions make a diagonal line in the ac plane may have affected the crystal-packing contraction along the a and c axes in the crystal compared with the typical crystal. |
==About this Structure== | ==About this Structure== | ||
| - | 2DE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http:// | + | 2DE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DE9 OCA]. |
==Reference== | ==Reference== | ||
| - | Tris(hydroxymethyl)aminomethane induces conformational change and crystal-packing contraction of porcine pancreatic elastase., Kinoshita T, Yamaguchi A, Tada T, Acta | + | Tris(hydroxymethyl)aminomethane induces conformational change and crystal-packing contraction of porcine pancreatic elastase., Kinoshita T, Yamaguchi A, Tada T, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt, 7):623-6. Epub 2006 Jun 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16820677 16820677] |
[[Category: Pancreatic elastase]] | [[Category: Pancreatic elastase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:57:53 2008'' |
Revision as of 14:57, 21 February 2008
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Crystal structure of porcine pancreatic elastase complexed with Tris after soaking a tris-free solution
Overview
Porcine pancreatic elastase (PPE) was crystallized under new sulfate-free conditions containing 0.3 M NaCl and 50 mM tris(hydroxymethyl)aminomethane-HCl at pH 7.0. The crystal structure determined at 1.5 angstroms resolution had a unique conformation in four regions which contained loop portions. A chloride ion was bound near the catalytic triad instead of the sulfate ion in PDB entry 1qnj, a typical PPE crystal structure. However, the chloride ion did not affect the configuration of the catalytic triad. A tris(hydroxymethyl)aminomethane (Tris) molecule was bound to the S4 and S5 subsites in place of the adjacent molecule in the 1qnj crystal and played a significant role in the structural change of the region. The distortion in this region may subsequently have induced conformational changes in the other three regions. The fact that Tris and these four regions make a diagonal line in the ac plane may have affected the crystal-packing contraction along the a and c axes in the crystal compared with the typical crystal.
About this Structure
2DE9 is a Single protein structure of sequence from Sus scrofa with as ligand. Active as Pancreatic elastase, with EC number 3.4.21.36 Full crystallographic information is available from OCA.
Reference
Tris(hydroxymethyl)aminomethane induces conformational change and crystal-packing contraction of porcine pancreatic elastase., Kinoshita T, Yamaguchi A, Tada T, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt, 7):623-6. Epub 2006 Jun 26. PMID:16820677
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