2erl
From Proteopedia
(New page: 200px<br /><applet load="2erl" size="450" color="white" frame="true" align="right" spinBox="true" caption="2erl, resolution 1.0Å" /> '''PHEROMONE ER-1 FROM''...) |
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| - | [[Image:2erl.jpg|left|200px]]<br /><applet load="2erl" size=" | + | [[Image:2erl.jpg|left|200px]]<br /><applet load="2erl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2erl, resolution 1.0Å" /> | caption="2erl, resolution 1.0Å" /> | ||
'''PHEROMONE ER-1 FROM'''<br /> | '''PHEROMONE ER-1 FROM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Four different phasing methods have been applied to the determination of | + | Four different phasing methods have been applied to the determination of the crystal structure of the 40 amino-acid mating pheromone of the unicellular ciliated protozoan Euplotes raikovi. The difficulties, failures and successes in attempts to solve the structure by: (1) molecular replacement, (2) direct phasing using the 'Shake and Bake' algorithm, (3) isomorphous replacement, and (4) multiple-wavelength anomalous dispersion are described. The structure was first solved by molecular replacement, and then was the first successful structure determination by 'Shake and Bake' without the direct involvement of its authors. A description of the current status of the high-resolution refinement of the structure is also given. The model is refined against 1 A resolution data to an R factor of 12.9%, and includes H atoms and discretely disordered side chains. |
==About this Structure== | ==About this Structure== | ||
| - | 2ERL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi] with EOH as [http://en.wikipedia.org/wiki/ligand ligand]. This structure | + | 2ERL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi] with <scene name='pdbligand=EOH:'>EOH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1ERL. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Euplotes raikovi]] | [[Category: Euplotes raikovi]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Anderson, D | + | [[Category: Anderson, D H.]] |
[[Category: Eisenberg, D.]] | [[Category: Eisenberg, D.]] | ||
| - | [[Category: Weiss, M | + | [[Category: Weiss, M S.]] |
[[Category: EOH]] | [[Category: EOH]] | ||
[[Category: pheromone]] | [[Category: pheromone]] | ||
[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:13:51 2008'' |
Revision as of 15:13, 21 February 2008
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PHEROMONE ER-1 FROM
Overview
Four different phasing methods have been applied to the determination of the crystal structure of the 40 amino-acid mating pheromone of the unicellular ciliated protozoan Euplotes raikovi. The difficulties, failures and successes in attempts to solve the structure by: (1) molecular replacement, (2) direct phasing using the 'Shake and Bake' algorithm, (3) isomorphous replacement, and (4) multiple-wavelength anomalous dispersion are described. The structure was first solved by molecular replacement, and then was the first successful structure determination by 'Shake and Bake' without the direct involvement of its authors. A description of the current status of the high-resolution refinement of the structure is also given. The model is refined against 1 A resolution data to an R factor of 12.9%, and includes H atoms and discretely disordered side chains.
About this Structure
2ERL is a Single protein structure of sequence from Euplotes raikovi with as ligand. This structure supersedes the now removed PDB entry 1ERL. Full crystallographic information is available from OCA.
Reference
A challenging case for protein crystal structure determination: the mating pheromone Er-1 from Euplotes raikovi., Anderson DH, Weiss MS, Eisenberg D, Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):469-80. PMID:15299668
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