2ey4
From Proteopedia
(New page: 200px<br /><applet load="2ey4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ey4, resolution 2.11Å" /> '''Crystal Structure of...) |
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| - | [[Image:2ey4.gif|left|200px]]<br /><applet load="2ey4" size=" | + | [[Image:2ey4.gif|left|200px]]<br /><applet load="2ey4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ey4, resolution 2.11Å" /> | caption="2ey4, resolution 2.11Å" /> | ||
'''Crystal Structure of a Cbf5-Nop10-Gar1 Complex'''<br /> | '''Crystal Structure of a Cbf5-Nop10-Gar1 Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide | + | H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide RNA, modify ribosomal and small nuclear RNAs. The H/ACA proteins are also essential components of telomerase in mammals. Cbf5 is the H/ACA protein that catalyzes isomerization of uridine to pseudouridine in target RNAs. Mutations in human Cbf5 (dyskerin) lead to dyskeratosis congenita. Here, we describe the 2.1 A crystal structure of a specific complex of three archaeal H/ACA proteins, Cbf5, Nop10, and Gar1. Cbf5 displays structural properties that are unique among known pseudouridine synthases and are consistent with its distinct function in RNA-guided pseudouridylation. We also describe the previously unknown structures of both Nop10 and Gar1 and the structural basis for their essential roles in pseudouridylation. By using information from related structures, we have modeled the entire ribonucleoprotein complex including both guide and substrate RNAs. We have also identified a dyskeratosis congenita mutation cluster site within a modeled dyskerin structure. |
==About this Structure== | ==About this Structure== | ||
| - | 2EY4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] and [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2EY4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] and [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EY4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Liang, B.]] | [[Category: Liang, B.]] | ||
[[Category: Rashid, R.]] | [[Category: Rashid, R.]] | ||
| - | [[Category: SECSG, Southeast | + | [[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
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[[Category: trimeric complex]] | [[Category: trimeric complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:15:43 2008'' |
Revision as of 15:15, 21 February 2008
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Crystal Structure of a Cbf5-Nop10-Gar1 Complex
Overview
H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide RNA, modify ribosomal and small nuclear RNAs. The H/ACA proteins are also essential components of telomerase in mammals. Cbf5 is the H/ACA protein that catalyzes isomerization of uridine to pseudouridine in target RNAs. Mutations in human Cbf5 (dyskerin) lead to dyskeratosis congenita. Here, we describe the 2.1 A crystal structure of a specific complex of three archaeal H/ACA proteins, Cbf5, Nop10, and Gar1. Cbf5 displays structural properties that are unique among known pseudouridine synthases and are consistent with its distinct function in RNA-guided pseudouridylation. We also describe the previously unknown structures of both Nop10 and Gar1 and the structural basis for their essential roles in pseudouridylation. By using information from related structures, we have modeled the entire ribonucleoprotein complex including both guide and substrate RNAs. We have also identified a dyskeratosis congenita mutation cluster site within a modeled dyskerin structure.
About this Structure
2EY4 is a Protein complex structure of sequences from Pyrococcus furiosus and Pyrococcus furiosus dsm 3638 with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita., Rashid R, Liang B, Baker DL, Youssef OA, He Y, Phipps K, Terns RM, Terns MP, Li H, Mol Cell. 2006 Jan 20;21(2):249-60. PMID:16427014
Page seeded by OCA on Thu Feb 21 17:15:43 2008
Categories: Protein complex | Pyrococcus furiosus | Pyrococcus furiosus dsm 3638 | Li, H. | Liang, B. | Rashid, R. | SECSG, Southeast Collaboratory for Structural Genomics. | ZN | Protein structure initiative | Psi | Secsg | Southeast collaboratory for structural genomics | Structural genomics | Trimeric complex
