2f66
From Proteopedia
(New page: 200px<br /><applet load="2f66" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f66, resolution 2.80Å" /> '''Structure of the ESC...) |
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| - | [[Image:2f66.gif|left|200px]]<br /><applet load="2f66" size=" | + | [[Image:2f66.gif|left|200px]]<br /><applet load="2f66" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2f66, resolution 2.80Å" /> | caption="2f66, resolution 2.80Å" /> | ||
'''Structure of the ESCRT-I endosomal trafficking complex'''<br /> | '''Structure of the ESCRT-I endosomal trafficking complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The endosomal sorting complex required for transport (ESCRT) complexes are | + | The endosomal sorting complex required for transport (ESCRT) complexes are central to receptor downregulation, lysosome biogenesis, and budding of HIV. The yeast ESCRT-I complex contains the Vps23, Vps28, and Vps37 proteins, and its assembly is directed by the C-terminal steadiness box of Vps23, the N-terminal half of Vps28, and the C-terminal half of Vps37. The crystal structures of a Vps23:Vps28 core subcomplex and the Vps23:Vps28:Vps37 core were solved at 2.1 and 2.8 A resolution. Each subunit contains a structurally similar pair of helices that form the core. The N-terminal domain of Vps28 has a hydrophobic binding site on its surface that is conformationally dynamic. The C-terminal domain of Vps28 binds the ESCRT-II complex. The structure shows how ESCRT-I is assembled by a compact core from which the Vps23 UEV domain, the Vps28 C domain, and other domains project to bind their partners. |
==About this Structure== | ==About this Structure== | ||
| - | 2F66 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2F66 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F66 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Hurley, J | + | [[Category: Hurley, J H.]] |
[[Category: Kim, J.]] | [[Category: Kim, J.]] | ||
| - | [[Category: Kostelansky, M | + | [[Category: Kostelansky, M S.]] |
[[Category: Lee, S.]] | [[Category: Lee, S.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: vps37]] | [[Category: vps37]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:08 2008'' |
Revision as of 15:18, 21 February 2008
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Structure of the ESCRT-I endosomal trafficking complex
Overview
The endosomal sorting complex required for transport (ESCRT) complexes are central to receptor downregulation, lysosome biogenesis, and budding of HIV. The yeast ESCRT-I complex contains the Vps23, Vps28, and Vps37 proteins, and its assembly is directed by the C-terminal steadiness box of Vps23, the N-terminal half of Vps28, and the C-terminal half of Vps37. The crystal structures of a Vps23:Vps28 core subcomplex and the Vps23:Vps28:Vps37 core were solved at 2.1 and 2.8 A resolution. Each subunit contains a structurally similar pair of helices that form the core. The N-terminal domain of Vps28 has a hydrophobic binding site on its surface that is conformationally dynamic. The C-terminal domain of Vps28 binds the ESCRT-II complex. The structure shows how ESCRT-I is assembled by a compact core from which the Vps23 UEV domain, the Vps28 C domain, and other domains project to bind their partners.
About this Structure
2F66 is a Protein complex structure of sequences from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Structural and functional organization of the ESCRT-I trafficking complex., Kostelansky MS, Sun J, Lee S, Kim J, Ghirlando R, Hierro A, Emr SD, Hurley JH, Cell. 2006 Apr 7;125(1):113-26. PMID:16615894
Page seeded by OCA on Thu Feb 21 17:18:08 2008
Categories: Protein complex | Saccharomyces cerevisiae | Hurley, J H. | Kim, J. | Kostelansky, M S. | Lee, S. | SO4 | Endosomal sorting complex required for transport | Endosome | Escrt protein complexes | Escrt-i | Trafficking complex | Tsg101 | Ubiquitin | Vacuolar protein sorting | Vps23 | Vps28 | Vps37
