2f8k
From Proteopedia
(New page: 200px<br /><applet load="2f8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f8k, resolution 2.000Å" /> '''Sequence specific r...) |
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| - | [[Image:2f8k.gif|left|200px]]<br /><applet load="2f8k" size=" | + | [[Image:2f8k.gif|left|200px]]<br /><applet load="2f8k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2f8k, resolution 2.000Å" /> | caption="2f8k, resolution 2.000Å" /> | ||
'''Sequence specific recognition of RNA hairpins by the SAM domain of Vts1'''<br /> | '''Sequence specific recognition of RNA hairpins by the SAM domain of Vts1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The SAM domain of the Saccharomyces cerevisiae post-transcriptional | + | The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1p epitomizes a subfamily of SAM domains conserved from yeast to humans that function as sequence-specific RNA-binding domains. Here we report the 2.0-A X-ray structure of the Vts1p SAM domain bound to a high-affinity RNA ligand. Specificity of RNA binding arises from the association of a guanosine loop base with a shallow pocket on the SAM domain and from multiple SAM domain contacts to the unique backbone structure of the loop, defined in part by a nonplanar base pair within the loop. We have validated NNF1 as an endogenous target of Vts1p among 79 transcripts that copurify with Vts1p. Bioinformatic analysis of these mRNAs demonstrates that the RNA-binding specificity of Vts1p in vivo is probably more stringent than that of the isolated SAM domain in vitro. |
==About this Structure== | ==About this Structure== | ||
| - | 2F8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2F8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lin, Z.]] | [[Category: Lin, Z.]] | ||
[[Category: Sicheri, F.]] | [[Category: Sicheri, F.]] | ||
| - | [[Category: Smibert, C | + | [[Category: Smibert, C A.]] |
[[Category: sam domain]] | [[Category: sam domain]] | ||
[[Category: vts1/rna complex]] | [[Category: vts1/rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:50 2008'' |
Revision as of 15:18, 21 February 2008
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Sequence specific recognition of RNA hairpins by the SAM domain of Vts1
Overview
The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1p epitomizes a subfamily of SAM domains conserved from yeast to humans that function as sequence-specific RNA-binding domains. Here we report the 2.0-A X-ray structure of the Vts1p SAM domain bound to a high-affinity RNA ligand. Specificity of RNA binding arises from the association of a guanosine loop base with a shallow pocket on the SAM domain and from multiple SAM domain contacts to the unique backbone structure of the loop, defined in part by a nonplanar base pair within the loop. We have validated NNF1 as an endogenous target of Vts1p among 79 transcripts that copurify with Vts1p. Bioinformatic analysis of these mRNAs demonstrates that the RNA-binding specificity of Vts1p in vivo is probably more stringent than that of the isolated SAM domain in vitro.
About this Structure
2F8K is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Sequence-specific recognition of RNA hairpins by the SAM domain of Vts1p., Aviv T, Lin Z, Ben-Ari G, Smibert CA, Sicheri F, Nat Struct Mol Biol. 2006 Feb;13(2):168-76. Epub 2006 Jan 22. PMID:16429151
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