2onq

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{{Seed}}
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==Gbeta1 stabilization by in vitro evolution and computational design==
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[[Image:2onq.png|left|200px]]
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<StructureSection load='2onq' size='340' side='right' caption='[[2onq]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2onq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._g148 Streptococcus sp. g148]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONQ OCA]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2on8|2on8]], [[1pga|1pga]], [[1gb4|1gb4]], [[1fcc|1fcc]], [[3gb1|3gb1]], [[1p7e|1p7e]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1324 Streptococcus sp. G148])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2onq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2onq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2onq RCSB], [http://www.ebi.ac.uk/pdbsum/2onq PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/2onq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Computational design and in vitro evolution are major strategies for stabilizing proteins. For the four critical positions 16, 18, 25, and 29 of the B domain of the streptococcal protein G (Gbeta1), they identified the same optimal residues at positions 16 and 25, but not at 18 and 29. Here we analyzed the energetic contributions of the residues from these two approaches by single and double mutant analyses and determined crystal structures for a variant from the calculation (I16/L18/E25/K29) and from the selection (I16/I18/E25/F29). The structural analysis explains the observed differences in stabilization. Residues 16, 18, and 29 line an invagination, which results from a packing defect between the helix and the beta-sheet of Gbeta1. In all stabilized variants, residues with larger side-chains occur at these positions and packing is improved. In the selected variant, packing is better optimized than in the computed variant. Such differences in side-chain packing strongly affect stability but are difficult to evaluate by computation.
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Optimization of the gbeta1 domain by computational design and by in vitro evolution: structural and energetic basis of stabilization.,Wunderlich M, Max KE, Roske Y, Mueller U, Heinemann U, Schmid FX J Mol Biol. 2007 Oct 26;373(3):775-84. Epub 2007 Aug 19. PMID:17868696<ref>PMID:17868696</ref>
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The line below this paragraph, containing "STRUCTURE_2onq", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_2onq| PDB=2onq | SCENE= }}
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===Gbeta1 stabilization by in vitro evolution and computational design===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_17868696}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 17868696 is the PubMed ID number.
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</StructureSection>
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[[Category: Streptococcus sp. g148]]
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{{ABSTRACT_PUBMED_17868696}}
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==About this Structure==
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2ONQ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp. Streptococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONQ OCA].
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==Reference==
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<ref group="xtra">PMID:17868696</ref><references group="xtra"/>
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[[Category: Streptococcus sp.]]
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[[Category: Heinemann, U.]]
[[Category: Heinemann, U.]]
[[Category: Max, K E.A.]]
[[Category: Max, K E.A.]]
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[[Category: Improved hydrophobic packing of core residue]]
[[Category: Improved hydrophobic packing of core residue]]
[[Category: Protein binding]]
[[Category: Protein binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:48:00 2009''
 

Revision as of 08:41, 7 May 2014

Gbeta1 stabilization by in vitro evolution and computational design

2onq, resolution 1.70Å

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