1bto
From Proteopedia
(New page: 200px<br /> <applet load="1bto" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bto, resolution 2.0Å" /> '''HORSE LIVER ALCOHOL ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BTO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]] with ZN, NAD and SSB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BTO OCA]]. | + | 1BTO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]] with ZN, NAD and SSB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1]]. Structure known Active Site: NAD. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BTO OCA]]. |
==Reference== | ==Reference== | ||
Flexibility of liver alcohol dehydrogenase in stereoselective binding of 3-butylthiolane 1-oxides., Cho H, Ramaswamy S, Plapp BV, Biochemistry. 1997 Jan 14;36(2):382-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9003191 9003191] | Flexibility of liver alcohol dehydrogenase in stereoselective binding of 3-butylthiolane 1-oxides., Cho H, Ramaswamy S, Plapp BV, Biochemistry. 1997 Jan 14;36(2):382-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9003191 9003191] | ||
| + | [[Category: Alcohol dehydrogenase]] | ||
[[Category: Equus caballus]] | [[Category: Equus caballus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sulfoxide]] | [[Category: sulfoxide]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:23:48 2007'' |
Revision as of 12:19, 30 October 2007
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HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED TO NADH AND (1S,3R)3-BUTYLTHIOLANE 1-OXIDE
Overview
Thiolane 1-oxides are analogs of the carbonyl substrates that bind to the, alcohol dehydrogenase-NADH complex and are potent uncompetitive inhibitors, against alcohol [Chadha, V. K., et al. (1985) J. Med. Chem. 28, 36-40]., The four stereoisomers of 3-butylthiolane 1-oxide (BTO) were separated by, chiral phase chromatography. CD and 1H-NMR spectra identified the, enantiomeric pairs. 1H-NMR chemical shifts were assigned on the basis of, COSY spectra of both diastereoisomers and confirmed by HMQC spectra., Coupling constants were determined through one-dimensional decoupling, experiments. NMR with chiral shift reagents, Eu(hfc)3 [europium tris, [3-[(heptafluoropropyl)hydroxymethylene]-(+)-camphorate]] or, (R)-(-)-N-(3,5-dinitrobenzoyl)-alpha-methylbenzylamine, determined that, the most ... [(full description)]
About this Structure
1BTO is a [Single protein] structure of sequence from [Equus caballus] with ZN, NAD and SSB as [ligands]. Active as [Alcohol dehydrogenase], with EC number [1.1.1.1]. Structure known Active Site: NAD. Full crystallographic information is available from [OCA].
Reference
Flexibility of liver alcohol dehydrogenase in stereoselective binding of 3-butylthiolane 1-oxides., Cho H, Ramaswamy S, Plapp BV, Biochemistry. 1997 Jan 14;36(2):382-9. PMID:9003191
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