2fp7
From Proteopedia
(New page: 200px<br /><applet load="2fp7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fp7, resolution 1.68Å" /> '''West Nile Virus NS2B...) |
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| - | [[Image:2fp7.gif|left|200px]]<br /><applet load="2fp7" size=" | + | [[Image:2fp7.gif|left|200px]]<br /><applet load="2fp7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fp7, resolution 1.68Å" /> | caption="2fp7, resolution 1.68Å" /> | ||
'''West Nile Virus NS2B/NS3protease in complex with Bz-Nle-Lys-Arg-Arg-H'''<br /> | '''West Nile Virus NS2B/NS3protease in complex with Bz-Nle-Lys-Arg-Arg-H'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The replication of flaviviruses requires the correct processing of their | + | The replication of flaviviruses requires the correct processing of their polyprotein by the viral NS3 protease (NS3pro). Essential for the activation of NS3pro is a 47-residue region of NS2B. Here we report the crystal structures of a dengue NS2B-NS3pro complex and a West Nile virus NS2B-NS3pro complex with a substrate-based inhibitor. These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation. |
==About this Structure== | ==About this Structure== | ||
| - | 2FP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/West_nile_virus West nile virus] with NDL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Flavivirin Flavivirin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.91 3.4.21.91] Full crystallographic information is available from [http:// | + | 2FP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/West_nile_virus West nile virus] with <scene name='pdbligand=NDL:'>NDL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Flavivirin Flavivirin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.91 3.4.21.91] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FP7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: West nile virus]] | [[Category: West nile virus]] | ||
| - | [[Category: Arcy, A | + | [[Category: Arcy, A D.]] |
[[Category: Erbel, P.]] | [[Category: Erbel, P.]] | ||
[[Category: Schiering, N.]] | [[Category: Schiering, N.]] | ||
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[[Category: substrate-based inhibitor]] | [[Category: substrate-based inhibitor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:23:41 2008'' |
Revision as of 15:23, 21 February 2008
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West Nile Virus NS2B/NS3protease in complex with Bz-Nle-Lys-Arg-Arg-H
Overview
The replication of flaviviruses requires the correct processing of their polyprotein by the viral NS3 protease (NS3pro). Essential for the activation of NS3pro is a 47-residue region of NS2B. Here we report the crystal structures of a dengue NS2B-NS3pro complex and a West Nile virus NS2B-NS3pro complex with a substrate-based inhibitor. These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation.
About this Structure
2FP7 is a Protein complex structure of sequences from West nile virus with as ligand. Active as Flavivirin, with EC number 3.4.21.91 Full crystallographic information is available from OCA.
Reference
Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus., Erbel P, Schiering N, D'Arcy A, Renatus M, Kroemer M, Lim SP, Yin Z, Keller TH, Vasudevan SG, Hommel U, Nat Struct Mol Biol. 2006 Apr;13(4):372-3. Epub 2006 Mar 12. PMID:16532006
Page seeded by OCA on Thu Feb 21 17:23:41 2008
