2fv4
From Proteopedia
(New page: 200px<br /><applet load="2fv4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fv4" /> '''NMR solution structure of the yeast kinetoch...) |
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| - | [[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size=" | + | [[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fv4" /> | caption="2fv4" /> | ||
'''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain'''<br /> | '''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during | + | The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore. |
==About this Structure== | ==About this Structure== | ||
| - | 2FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Chou, J | + | [[Category: Chou, J J.]] |
| - | [[Category: Schnell, J | + | [[Category: Schnell, J R.]] |
[[Category: alpha-beta]] | [[Category: alpha-beta]] | ||
[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
[[Category: complex]] | [[Category: complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:23 2008'' |
Revision as of 15:25, 21 February 2008
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NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain
Overview
The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.
About this Structure
2FV4 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain., Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC, Structure. 2006 Jun;14(6):1003-9. PMID:16765893
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