2fwm
From Proteopedia
(New page: 200px<br /><applet load="2fwm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fwm, resolution 2.00Å" /> '''Crystal Structure of...) |
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| - | [[Image:2fwm.gif|left|200px]]<br /><applet load="2fwm" size=" | + | [[Image:2fwm.gif|left|200px]]<br /><applet load="2fwm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fwm, resolution 2.00Å" /> | caption="2fwm, resolution 2.00Å" /> | ||
'''Crystal Structure of E. coli EntA, a 2,3-dihydrodihydroxy benzoate dehydrogenase'''<br /> | '''Crystal Structure of E. coli EntA, a 2,3-dihydrodihydroxy benzoate dehydrogenase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Escherichia coli enterobactin synthetic cluster is composed of six | + | The Escherichia coli enterobactin synthetic cluster is composed of six proteins, EntA-EntF, that form the enterobactin molecule from three serine molecules and three molecules of 2,3-dihydroxybenzoic acid (DHB). EntC, EntB and EntA catalyze the three-step synthesis of DHB from chorismate. EntA is a member of the short-chain oxidoreductase (SCOR) family of proteins and catalyzes the final step in DHB synthesis, the NAD+-dependent oxidation of 2,3-dihydro-2,3-dihydroxybenzoic acid to DHB. The structure of EntA has been determined by multi-wavelength anomalous dispersion methods. Here, the 2.0 A crystal structure of EntA in the unliganded form is presented. Analysis of the structure in light of recent structural and bioinformatic analysis of other members of the SCOR family provides insight into the residues involved in cofactor and substrate binding. |
==About this Structure== | ==About this Structure== | ||
| - | 2FWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/2,3-dihydro-2,3-dihydroxybenzoate_dehydrogenase 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.28 1.3.1.28] Full crystallographic information is available from [http:// | + | 2FWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/2,3-dihydro-2,3-dihydroxybenzoate_dehydrogenase 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.28 1.3.1.28] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Duax, W | + | [[Category: Duax, W L.]] |
| - | [[Category: Gulick, A | + | [[Category: Gulick, A M.]] |
[[Category: chorismate metabolism]] | [[Category: chorismate metabolism]] | ||
[[Category: enterobactin]] | [[Category: enterobactin]] | ||
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[[Category: tetramer]] | [[Category: tetramer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:52 2008'' |
Revision as of 15:25, 21 February 2008
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Crystal Structure of E. coli EntA, a 2,3-dihydrodihydroxy benzoate dehydrogenase
Overview
The Escherichia coli enterobactin synthetic cluster is composed of six proteins, EntA-EntF, that form the enterobactin molecule from three serine molecules and three molecules of 2,3-dihydroxybenzoic acid (DHB). EntC, EntB and EntA catalyze the three-step synthesis of DHB from chorismate. EntA is a member of the short-chain oxidoreductase (SCOR) family of proteins and catalyzes the final step in DHB synthesis, the NAD+-dependent oxidation of 2,3-dihydro-2,3-dihydroxybenzoic acid to DHB. The structure of EntA has been determined by multi-wavelength anomalous dispersion methods. Here, the 2.0 A crystal structure of EntA in the unliganded form is presented. Analysis of the structure in light of recent structural and bioinformatic analysis of other members of the SCOR family provides insight into the residues involved in cofactor and substrate binding.
About this Structure
2FWM is a Single protein structure of sequence from Escherichia coli. Active as 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, with EC number 1.3.1.28 Full crystallographic information is available from OCA.
Reference
Determination of the crystal structure of EntA, a 2,3-dihydro-2,3-dihydroxybenzoic acid dehydrogenase from Escherichia coli., Sundlov JA, Garringer JA, Carney JM, Reger AS, Drake EJ, Duax WL, Gulick AM, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):734-40. Epub 2006, Jun 20. PMID:16790929
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