This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2g0k
From Proteopedia
(New page: 200px<br /><applet load="2g0k" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g0k" /> '''Solution Structure of Neocarzinostatin Apo-P...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2g0k.gif|left|200px]]<br /><applet load="2g0k" size=" | + | [[Image:2g0k.gif|left|200px]]<br /><applet load="2g0k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2g0k" /> | caption="2g0k" /> | ||
'''Solution Structure of Neocarzinostatin Apo-Protein'''<br /> | '''Solution Structure of Neocarzinostatin Apo-Protein'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2G0K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full crystallographic information is available from [http:// | + | 2G0K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0K OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Streptomyces carzinostaticus]] | [[Category: Streptomyces carzinostaticus]] | ||
[[Category: Caddick, S.]] | [[Category: Caddick, S.]] | ||
| - | [[Category: Muskett, F | + | [[Category: Muskett, F W.]] |
| - | [[Category: Stoneman, R | + | [[Category: Stoneman, R G.]] |
| - | [[Category: Woolfson, D | + | [[Category: Woolfson, D N.]] |
[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:26:57 2008'' |
Revision as of 15:26, 21 February 2008
|
Solution Structure of Neocarzinostatin Apo-Protein
Overview
Neocarzinostatin (NCS) is a 1:1 complex of an enediyne chromophore (NCSChrom), non-covalently bound to an 11 kDa protein (apoNCS). We are exploring apoNCS as a generic protein system for sequestering small molecules for therapeutic applications. Here, we disclose a new flavone ligand 1 for apoNCS and present a high-resolution NMR structure of this ligand bound to apoNCS. This is the first high-resolution structure of a completely non-cognate ligand bound to the apoNCS protein. This work provides unambiguous evidence that a completely new class of ligand can bind specifically to apoNCS. Furthermore, the mode of binding is different than that of the naphthoate-based ligands, and for such a simple hydrophobic compound, the new ligand surprisingly binds specifically. This work indicates that apo-Neocarzinostatin has multiple selective and distinct binding modes for small-molecule cargo.
About this Structure
2G0K is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Synthetic ligands for apo-neocarzinostatin., Caddick S, Muskett FW, Stoneman RG, Woolfson DN, J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:16568976
Page seeded by OCA on Thu Feb 21 17:26:57 2008
