2g5i
From Proteopedia
(New page: 200px<br /><applet load="2g5i" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g5i, resolution 3.35Å" /> '''Structure of tRNA-De...) |
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| - | [[Image:2g5i.gif|left|200px]]<br /><applet load="2g5i" size=" | + | [[Image:2g5i.gif|left|200px]]<br /><applet load="2g5i" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2g5i, resolution 3.35Å" /> | caption="2g5i, resolution 3.35Å" /> | ||
'''Structure of tRNA-Dependent Amidotransferase GatCAB complexed with ADP-AlF4'''<br /> | '''Structure of tRNA-Dependent Amidotransferase GatCAB complexed with ADP-AlF4'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the | + | The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-tRNA(Gln). |
==About this Structure== | ==About this Structure== | ||
| - | 2G5I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2G5I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: multi protein complex]] | [[Category: multi protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:28:21 2008'' |
Revision as of 15:28, 21 February 2008
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Structure of tRNA-Dependent Amidotransferase GatCAB complexed with ADP-AlF4
Overview
The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-tRNA(Gln).
About this Structure
2G5I is a Protein complex structure of sequences from Staphylococcus aureus with and as ligands. Full crystallographic information is available from OCA.
Reference
Ammonia channel couples glutaminase with transamidase reactions in GatCAB., Nakamura A, Yao M, Chimnaronk S, Sakai N, Tanaka I, Science. 2006 Jun 30;312(5782):1954-8. PMID:16809541
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