2gic
From Proteopedia
(New page: 200px<br /><applet load="2gic" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gic, resolution 2.920Å" /> '''Crystal Structure o...) |
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| - | [[Image:2gic.gif|left|200px]]<br /><applet load="2gic" size=" | + | [[Image:2gic.gif|left|200px]]<br /><applet load="2gic" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gic, resolution 2.920Å" /> | caption="2gic, resolution 2.920Å" /> | ||
'''Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex'''<br /> | '''Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Vesicular stomatitis virus is a negative-stranded RNA virus. Its | + | Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary. |
==About this Structure== | ==About this Structure== | ||
| - | 2GIC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus] with IUM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GIC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus] with <scene name='pdbligand=IUM:'>IUM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vesicular stomatitis indiana virus]] | [[Category: Vesicular stomatitis indiana virus]] | ||
| - | [[Category: Green, T | + | [[Category: Green, T J.]] |
[[Category: Luo, M.]] | [[Category: Luo, M.]] | ||
| - | [[Category: Wertz, G | + | [[Category: Wertz, G W.]] |
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
[[Category: IUM]] | [[Category: IUM]] | ||
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[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:31:57 2008'' |
Revision as of 15:31, 21 February 2008
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Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex
Overview
Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary.
About this Structure
2GIC is a Single protein structure of sequence from Vesicular stomatitis indiana virus with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the vesicular stomatitis virus nucleoprotein-RNA complex., Green TJ, Zhang X, Wertz GW, Luo M, Science. 2006 Jul 21;313(5785):357-60. Epub 2006 Jun 15. PMID:16778022
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