2gv5
From Proteopedia
(New page: 200px<br /><applet load="2gv5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gv5, resolution 3.00Å" /> '''crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2gv5.gif|left|200px]]<br /><applet load="2gv5" size=" | + | [[Image:2gv5.gif|left|200px]]<br /><applet load="2gv5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gv5, resolution 3.00Å" /> | caption="2gv5, resolution 3.00Å" /> | ||
'''crystal structure of Sfi1p/Cdc31p complex'''<br /> | '''crystal structure of Sfi1p/Cdc31p complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Centrins are calmodulin-like proteins present in centrosomes and yeast | + | Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly. |
==About this Structure== | ==About this Structure== | ||
| - | 2GV5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2GV5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GV5 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Conduit, P | + | [[Category: Conduit, P T.]] |
| - | [[Category: Kilmartin, J | + | [[Category: Kilmartin, J V.]] |
[[Category: Li, S.]] | [[Category: Li, S.]] | ||
| - | [[Category: Robinson, C | + | [[Category: Robinson, C V.]] |
| - | [[Category: Sandercock, A | + | [[Category: Sandercock, A M.]] |
| - | [[Category: Williams, R | + | [[Category: Williams, R L.]] |
[[Category: cdc31p]] | [[Category: cdc31p]] | ||
[[Category: centrin]] | [[Category: centrin]] | ||
| Line 25: | Line 25: | ||
[[Category: spindle pole body]] | [[Category: spindle pole body]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:35:44 2008'' |
Revision as of 15:35, 21 February 2008
|
crystal structure of Sfi1p/Cdc31p complex
Overview
Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.
About this Structure
2GV5 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:16785321
Page seeded by OCA on Thu Feb 21 17:35:44 2008
