2gwk
From Proteopedia
(New page: 200px<br /><applet load="2gwk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gwk, resolution 2.000Å" /> '''SpvB ADP-ribosylate...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2gwk.gif|left|200px]]<br /><applet load="2gwk" size=" | + | [[Image:2gwk.gif|left|200px]]<br /><applet load="2gwk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gwk, resolution 2.000Å" /> | caption="2gwk, resolution 2.000Å" /> | ||
'''SpvB ADP-ribosylated actin: orthorhombic crystal form'''<br /> | '''SpvB ADP-ribosylated actin: orthorhombic crystal form'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB | + | Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts. |
==About this Structure== | ==About this Structure== | ||
| - | 2GWK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with CA and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2GWK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWK OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Margarit, S | + | [[Category: Margarit, S M.]] |
| - | [[Category: Stebbins, C | + | [[Category: Stebbins, C E.]] |
[[Category: ATP]] | [[Category: ATP]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| Line 20: | Line 20: | ||
[[Category: adp-ribosylation]] | [[Category: adp-ribosylation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:05 2008'' |
Revision as of 15:36, 21 February 2008
|
SpvB ADP-ribosylated actin: orthorhombic crystal form
Overview
Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.
About this Structure
2GWK is a Single protein structure of sequence from Oryctolagus cuniculus with and as ligands. Full crystallographic information is available from OCA.
Reference
A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:16905096
Page seeded by OCA on Thu Feb 21 17:36:05 2008
