2h5f
From Proteopedia
(New page: 200px<br /><applet load="2h5f" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h5f, resolution 1.90Å" /> '''Denmotoxin: A the th...) |
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| - | [[Image:2h5f.gif|left|200px]]<br /><applet load="2h5f" size=" | + | [[Image:2h5f.gif|left|200px]]<br /><applet load="2h5f" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2h5f, resolution 1.90Å" /> | caption="2h5f, resolution 1.90Å" /> | ||
'''Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity'''<br /> | '''Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Boiga dendrophila (mangrove catsnake) is a colubrid snake that lives in | + | Boiga dendrophila (mangrove catsnake) is a colubrid snake that lives in Southeast Asian lowland rainforests and mangrove swamps and that preys primarily on birds. We have isolated, purified, and sequenced a novel toxin from its venom, which we named denmotoxin. It is a monomeric polypeptide of 77 amino acid residues with five disulfide bridges. In organ bath experiments, it displayed potent postsynaptic neuromuscular activity and irreversibly inhibited indirectly stimulated twitches in chick biventer cervicis nerve-muscle preparations. In contrast, it induced much smaller and readily reversible inhibition of electrically induced twitches in mouse hemidiaphragm nerve-muscle preparations. More precisely, the chick muscle alpha(1)betagammadelta-nicotinic acetylcholine receptor was 100-fold more susceptible compared with the mouse receptor. These data indicate that denmotoxin has a bird-specific postsynaptic activity. We chemically synthesized denmotoxin, crystallized it, and solved its crystal structure at 1.9 A by the molecular replacement method. The toxin structure adopts a non-conventional three-finger fold with an additional (fifth) disulfide bond in the first loop and seven additional residues at its N terminus, which is blocked by a pyroglutamic acid residue. This is the first crystal structure of a three-finger toxin from colubrid snake venom and the first fully characterized bird-specific toxin. Denmotoxin illustrates the relationship between toxin specificity and the primary prey type that constitutes the snake's diet. |
==About this Structure== | ==About this Structure== | ||
| - | 2H5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Boiga_dendrophila Boiga dendrophila] with PO4, K and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2H5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Boiga_dendrophila Boiga dendrophila] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Boiga dendrophila]] | [[Category: Boiga dendrophila]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kini, R | + | [[Category: Kini, R M.]] |
[[Category: Pawlak, J.]] | [[Category: Pawlak, J.]] | ||
| - | [[Category: Stura, E | + | [[Category: Stura, E A.]] |
[[Category: K]] | [[Category: K]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: three-finger toxin]] | [[Category: three-finger toxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:38:20 2008'' |
Revision as of 15:38, 21 February 2008
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Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity
Overview
Boiga dendrophila (mangrove catsnake) is a colubrid snake that lives in Southeast Asian lowland rainforests and mangrove swamps and that preys primarily on birds. We have isolated, purified, and sequenced a novel toxin from its venom, which we named denmotoxin. It is a monomeric polypeptide of 77 amino acid residues with five disulfide bridges. In organ bath experiments, it displayed potent postsynaptic neuromuscular activity and irreversibly inhibited indirectly stimulated twitches in chick biventer cervicis nerve-muscle preparations. In contrast, it induced much smaller and readily reversible inhibition of electrically induced twitches in mouse hemidiaphragm nerve-muscle preparations. More precisely, the chick muscle alpha(1)betagammadelta-nicotinic acetylcholine receptor was 100-fold more susceptible compared with the mouse receptor. These data indicate that denmotoxin has a bird-specific postsynaptic activity. We chemically synthesized denmotoxin, crystallized it, and solved its crystal structure at 1.9 A by the molecular replacement method. The toxin structure adopts a non-conventional three-finger fold with an additional (fifth) disulfide bond in the first loop and seven additional residues at its N terminus, which is blocked by a pyroglutamic acid residue. This is the first crystal structure of a three-finger toxin from colubrid snake venom and the first fully characterized bird-specific toxin. Denmotoxin illustrates the relationship between toxin specificity and the primary prey type that constitutes the snake's diet.
About this Structure
2H5F is a Single protein structure of sequence from Boiga dendrophila with , and as ligands. Full crystallographic information is available from OCA.
Reference
Denmotoxin, a three-finger toxin from the colubrid snake Boiga dendrophila (Mangrove Catsnake) with bird-specific activity., Pawlak J, Mackessy SP, Fry BG, Bhatia M, Mourier G, Fruchart-Gaillard C, Servent D, Menez R, Stura E, Menez A, Kini RM, J Biol Chem. 2006 Sep 29;281(39):29030-41. Epub 2006 Jul 24. PMID:16864572
Page seeded by OCA on Thu Feb 21 17:38:20 2008
Categories: Boiga dendrophila | Single protein | Kini, R M. | Pawlak, J. | Stura, E A. | K | NA | PO4 | Neurotoxin | Snake venom | Three-finger toxin
