2h5x
From Proteopedia
(New page: 200px<br /><applet load="2h5x" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h5x, resolution 2.70Å" /> '''RuvA from Mycobacter...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2h5x.gif|left|200px]]<br /><applet load="2h5x" size=" | + | [[Image:2h5x.gif|left|200px]]<br /><applet load="2h5x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2h5x, resolution 2.70Å" /> | caption="2h5x, resolution 2.70Å" /> | ||
'''RuvA from Mycobacterium tuberculosis'''<br /> | '''RuvA from Mycobacterium tuberculosis'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The process of recombinational repair is crucial for maintaining genomic | + | The process of recombinational repair is crucial for maintaining genomic integrity and generating biological diversity. In association with RuvB and RuvC, RuvA plays a central role in processing and resolving Holliday junctions, which are a critical intermediate in homologous recombination. Here, the cloning, purification and structure determination of the RuvA protein from Mycobacterium tuberculosis (MtRuvA) are reported. Analysis of the structure and comparison with other known RuvA proteins reveal an octameric state with conserved subunit-subunit interaction surfaces, indicating the requirement of octamer formation for biological activity. A detailed analysis of plasticity in the RuvA molecules has led to insights into the invariant and variable regions, thus providing a framework for understanding regional flexibility in various aspects of RuvA function. |
==About this Structure== | ==About this Structure== | ||
| - | 2H5X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2H5X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5X OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of Mycobacterium tuberculosis RuvA, a protein involved in recombination., Prabu JR, Thamotharan S, Khanduja JS, Alipio EZ, Kim CY, Waldo GS, Terwilliger TC, Segelke B, Lekin T, Toppani D, Hung LW, Yu M, Bursey E, Muniyappa K, Chandra NR, Vijayan M, Acta | + | Structure of Mycobacterium tuberculosis RuvA, a protein involved in recombination., Prabu JR, Thamotharan S, Khanduja JS, Alipio EZ, Kim CY, Waldo GS, Terwilliger TC, Segelke B, Lekin T, Toppani D, Hung LW, Yu M, Bursey E, Muniyappa K, Chandra NR, Vijayan M, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):731-4. Epub 2006 Jul 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16880543 16880543] |
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alipio, E | + | [[Category: Alipio, E Z.]] |
[[Category: Bursey, E.]] | [[Category: Bursey, E.]] | ||
| - | [[Category: Chandra, N | + | [[Category: Chandra, N R.]] |
| - | [[Category: Hung, L | + | [[Category: Hung, L W.]] |
| - | [[Category: Khanduja, J | + | [[Category: Khanduja, J S.]] |
| - | [[Category: Kim, C | + | [[Category: Kim, C Y.]] |
[[Category: Lekin, T.]] | [[Category: Lekin, T.]] | ||
[[Category: Muniyappa, K.]] | [[Category: Muniyappa, K.]] | ||
| - | [[Category: Prabu, J | + | [[Category: Prabu, J R.]] |
[[Category: Segelke, B.]] | [[Category: Segelke, B.]] | ||
| - | [[Category: Terwilliger, T | + | [[Category: Terwilliger, T C.]] |
[[Category: Thamotharan, S.]] | [[Category: Thamotharan, S.]] | ||
[[Category: Toppani, D.]] | [[Category: Toppani, D.]] | ||
[[Category: Vijayan, M.]] | [[Category: Vijayan, M.]] | ||
| - | [[Category: Waldo, G | + | [[Category: Waldo, G S.]] |
[[Category: Yu, M.]] | [[Category: Yu, M.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
| Line 34: | Line 34: | ||
[[Category: ruva]] | [[Category: ruva]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:38:29 2008'' |
Revision as of 15:38, 21 February 2008
|
RuvA from Mycobacterium tuberculosis
Overview
The process of recombinational repair is crucial for maintaining genomic integrity and generating biological diversity. In association with RuvB and RuvC, RuvA plays a central role in processing and resolving Holliday junctions, which are a critical intermediate in homologous recombination. Here, the cloning, purification and structure determination of the RuvA protein from Mycobacterium tuberculosis (MtRuvA) are reported. Analysis of the structure and comparison with other known RuvA proteins reveal an octameric state with conserved subunit-subunit interaction surfaces, indicating the requirement of octamer formation for biological activity. A detailed analysis of plasticity in the RuvA molecules has led to insights into the invariant and variable regions, thus providing a framework for understanding regional flexibility in various aspects of RuvA function.
About this Structure
2H5X is a Single protein structure of sequence from Mycobacterium tuberculosis with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of Mycobacterium tuberculosis RuvA, a protein involved in recombination., Prabu JR, Thamotharan S, Khanduja JS, Alipio EZ, Kim CY, Waldo GS, Terwilliger TC, Segelke B, Lekin T, Toppani D, Hung LW, Yu M, Bursey E, Muniyappa K, Chandra NR, Vijayan M, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):731-4. Epub 2006 Jul 24. PMID:16880543
Page seeded by OCA on Thu Feb 21 17:38:29 2008
Categories: Mycobacterium tuberculosis | Single protein | Alipio, E Z. | Bursey, E. | Chandra, N R. | Hung, L W. | Khanduja, J S. | Kim, C Y. | Lekin, T. | Muniyappa, K. | Prabu, J R. | Segelke, B. | Terwilliger, T C. | Thamotharan, S. | Toppani, D. | Vijayan, M. | Waldo, G S. | Yu, M. | GOL | Holliday junction binding | Recombination | Ruva
