2bnj
From Proteopedia
(New page: 200px<br /> <applet load="2bnj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bnj, resolution 1.600Å" /> '''THE XYLANASE TA FR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BNJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]] with FER as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BNJ OCA]]. | + | 2BNJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]] with FER as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BNJ OCA]]. |
==Reference== | ==Reference== | ||
A family 10 Thermoascus aurantiacus xylanase utilizes arabinose decorations of xylan as significant substrate specificity determinants., Vardakou M, Flint J, Christakopoulos P, Lewis RJ, Gilbert HJ, Murray JW, J Mol Biol. 2005 Oct 7;352(5):1060-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16140328 16140328] | A family 10 Thermoascus aurantiacus xylanase utilizes arabinose decorations of xylan as significant substrate specificity determinants., Vardakou M, Flint J, Christakopoulos P, Lewis RJ, Gilbert HJ, Murray JW, J Mol Biol. 2005 Oct 7;352(5):1060-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16140328 16140328] | ||
| + | [[Category: Endo-1,4-beta-xylanase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermoascus aurantiacus]] | [[Category: Thermoascus aurantiacus]] | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:29:06 2007'' |
Revision as of 12:24, 30 October 2007
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THE XYLANASE TA FROM THERMOASCUS AURANTIACUS UTILIZES ARABINOSE DECORATIONS OF XYLAN AS SIGNIFICANT SUBSTRATE SPECIFICITY DETERMINANTS.
Overview
Xylan, which is a key component of the plant cell wall, consists of a, backbone of beta-1,4-linked xylose residues that are decorated with, arabinofuranose, acetyl, 4-O-methyl d-glucuronic acid and ferulate. The, backbone of xylan is hydrolysed by endo-beta1,4-xylanases (xylanases);, however, it is unclear whether the various side-chains of the, polysaccharide are utilized by these enzymes as significant substrate, specificity determinants. To address this question we have determined the, crystal structure of a family 10 xylanase from Thermoascus aurantiacus, in, complex with xylobiose containing an arabinofuranosyl-ferulate side-chain., We show that the distal glycone subsite of the enzyme makes extensive, direct and indirect interactions with the arabinose side-chain, while the, ... [(full description)]
About this Structure
2BNJ is a [Single protein] structure of sequence from [Thermoascus aurantiacus] with FER as [ligand]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
A family 10 Thermoascus aurantiacus xylanase utilizes arabinose decorations of xylan as significant substrate specificity determinants., Vardakou M, Flint J, Christakopoulos P, Lewis RJ, Gilbert HJ, Murray JW, J Mol Biol. 2005 Oct 7;352(5):1060-7. PMID:16140328
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