2h8e
From Proteopedia
(New page: 200px<br /><applet load="2h8e" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h8e, resolution 1.20Å" /> '''Structure RusA D70N'...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2h8e.jpg|left|200px]]<br /><applet load="2h8e" size=" | + | [[Image:2h8e.jpg|left|200px]]<br /><applet load="2h8e" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2h8e, resolution 1.20Å" /> | caption="2h8e, resolution 1.20Å" /> | ||
'''Structure RusA D70N'''<br /> | '''Structure RusA D70N'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2H8E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2H8E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8E OCA]. |
==Reference== | ==Reference== | ||
| Line 18: | Line 18: | ||
[[Category: resolvase]] | [[Category: resolvase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:34:52 2008'' |
Revision as of 13:34, 23 January 2008
|
Structure RusA D70N
Overview
We have determined the structure of a catalytically inactive D70N variant, of the Escherichia coli RusA resolvase bound to a duplex DNA substrate, that reveals critical protein-DNA interactions and permits a much clearer, understanding of the interaction of the enzyme with a Holliday junction, (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by, homologous recombination, by introducing symmetrical cuts in the, phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows, a high level of selectivity for DNA junctions, preferring to bind fourway, junctions over other substrates in vitro, it has also been shown to have, appreciable affinity for duplex DNA. However, RusA does not show DNA, cleavage activity with duplex substrates. Our structure suggests the, possible basis for structural selectivity as well as sources of the, sequence specificity observed for DNA cleavage by RusA.
About this Structure
2H8E is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity., Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG, Rafferty JB, Nucleic Acids Res. 2006;34(19):5577-84. Epub 2006 Oct 5. PMID:17028102
Page seeded by OCA on Wed Jan 23 15:34:52 2008
