2hb0
From Proteopedia
(New page: 200px<br /><applet load="2hb0" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hb0, resolution 2.30Å" /> '''Crystal Structure of...) |
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| - | [[Image:2hb0.gif|left|200px]]<br /><applet load="2hb0" size=" | + | [[Image:2hb0.gif|left|200px]]<br /><applet load="2hb0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hb0, resolution 2.30Å" /> | caption="2hb0, resolution 2.30Å" /> | ||
'''Crystal Structure of CfaE, the Adhesive Subunit of CFA/I Fimbria of Enterotoxigenic Escherichia coli'''<br /> | '''Crystal Structure of CfaE, the Adhesive Subunit of CFA/I Fimbria of Enterotoxigenic Escherichia coli'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HB0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MLI and PEG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2HB0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MLI:'>MLI</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HB0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: traveler's disease]] | [[Category: traveler's disease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:02:16 2008'' |
Revision as of 10:02, 23 January 2008
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Crystal Structure of CfaE, the Adhesive Subunit of CFA/I Fimbria of Enterotoxigenic Escherichia coli
Overview
CfaE is the minor, tip-localized adhesive subunit of colonization factor, antigen I fimbriae (CFA/I) of enterotoxigenic E. coli (ETEC) and is, thought to be essential for the attachment of ETEC to the human small, intestine early in diarrhea pathogenesis. The crystal structure of an in, cis donor strand complemented CfaE (dscCfaE) was determined, providing the, first atomic view of a fimbrial subunit assembled by the alternate, chaperone pathway. The dscCfaE structure consists of an N-terminal adhesin, domain and a C-terminal pilin domain of similar size, each featuring a, variable immunoglobulin-like fold. Extensive interactions exist between, the two domains and appear to rigidify the molecule. The upper surface of, the adhesin domain distal to the pilin domain reveals a depression, consisting of conserved residues including Arg181, previously shown to be, necessary for erythrocyte adhesion. Mutational analysis revealed a cluster, of conserved, positively charged residues that are required for CFA/I, mediated hemagglutination, implicating this as the receptor-binding, pocket. Mutations in a few subclass-specific residues that surround the, cluster displayed differential effects on the two red cell species used in, hemagglutination, suggesting that these residues play a role in host or, cell specificity. The C-terminal donor strand derived from the major, subunit CfaB is folded as a beta-strand and fits into a hydrophobic groove, in the pilin domain to complete the immunoglobulin fold. The location of, this well-ordered donor strand suggests the positioning and orientation of, the subjacent major fimbrial subunit CfaB in the native assembly of CFA/I, fimbriae.
About this Structure
2HB0 is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
Reference
A receptor-binding site as revealed by the crystal structure of CfaE,the CFA/I fimbrial adhesin of enterotoxigenic Escherichia coli., Li YF, Poole S, Rasulova F, McVeigh AL, Savarino SJ, Xia D, J Biol Chem. 2007 Jun 14;. PMID:17569668
Page seeded by OCA on Wed Jan 23 12:02:16 2008
Categories: Escherichia coli | Single protein | Li, Y.F. | Poole, S. | Rasulova, F. | Savarino, S.J. | Xia, D. | MLI | PEG | Adhesin | Cell adhesion | Cfa/i | Cfae | Etec | Traveler's disease
