2hcy
From Proteopedia
(New page: 200px<br /><applet load="2hcy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hcy, resolution 2.44Å" /> '''Yeast Alcohol Dehydr...) |
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| - | [[Image:2hcy.gif|left|200px]]<br /><applet load="2hcy" size=" | + | [[Image:2hcy.gif|left|200px]]<br /><applet load="2hcy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hcy, resolution 2.44Å" /> | caption="2hcy, resolution 2.44Å" /> | ||
'''Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme'''<br /> | '''Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN, 8ID and ETF as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] Full crystallographic information is available from [http:// | + | 2HCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=8ID:'>8ID</scene> and <scene name='pdbligand=ETF:'>ETF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HCY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc coordination]] | [[Category: zinc coordination]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:48:46 2008'' |
Revision as of 10:48, 23 January 2008
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Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme
Overview
The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C, as hexagonal plates or short columns in the presence of NAD+ and, 2,2,2-trifluoroethanol, in sodium, N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to, 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data, to 3.2 A resolution show that the crystals are hexagonal in space group, P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one, subunit of the tetrameric enzyme per asymmetric unit, giving a packing, density of 2.9 A3/Da.
About this Structure
2HCY is a Single protein structure of sequence from Saccharomyces cerevisiae with , and as ligands. Active as Alcohol dehydrogenase, with EC number 1.1.1.1 Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I., Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV, J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:8289298
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