2hl6
From Proteopedia
(New page: 200px<br /><applet load="2hl6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hl6, resolution 1.550Å" /> '''Structure of homolo...) |
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| - | [[Image:2hl6.gif|left|200px]]<br /><applet load="2hl6" size=" | + | [[Image:2hl6.gif|left|200px]]<br /><applet load="2hl6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hl6, resolution 1.550Å" /> | caption="2hl6, resolution 1.550Å" /> | ||
'''Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS'''<br /> | '''Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied. | + | The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied. From the most to the least thermo-resistant, the four molecular species ranked as follows: (i) glycosylated form produced native, (ii) non-glycosylated form produced native, (iii) non-glycosylated form produced as inclusion bodies and refolded, and (iv) glycosylated form produced native chemically denatured and then refolded. On the basis of these results and of crystal structure data, we discuss the respective importance of protein folding and glycosylation in the thermal stability of recombinant FAEA. |
==About this Structure== | ==About this Structure== | ||
| - | 2HL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with SO4, EDO and CXS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] Full crystallographic information is available from [http:// | + | 2HL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=CXS:'>CXS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: glycosylated]] | [[Category: glycosylated]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:03 2008'' |
Revision as of 15:43, 21 February 2008
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Structure of homologously expressed Ferrulate esterase of Aspergillus niger in complex with CAPS
Overview
The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied. From the most to the least thermo-resistant, the four molecular species ranked as follows: (i) glycosylated form produced native, (ii) non-glycosylated form produced native, (iii) non-glycosylated form produced as inclusion bodies and refolded, and (iv) glycosylated form produced native chemically denatured and then refolded. On the basis of these results and of crystal structure data, we discuss the respective importance of protein folding and glycosylation in the thermal stability of recombinant FAEA.
About this Structure
2HL6 is a Single protein structure of sequence from Aspergillus niger with , and as ligands. Active as Feruloyl esterase, with EC number 3.1.1.73 Full crystallographic information is available from OCA.
Reference
Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A., Benoit I, Asther M, Sulzenbacher G, Record E, Marmuse L, Parsiegla G, Gimbert I, Asther M, Bignon C, FEBS Lett. 2006 Oct 30;580(25):5815-21. Epub 2006 Sep 27. PMID:17027758
Page seeded by OCA on Thu Feb 21 17:43:03 2008
Categories: Aspergillus niger | Feruloyl esterase | Single protein | Dubots, J. | Herpoel-Gimbert, I. | Parsiegla, G. | CXS | EDO | SO4 | A/b hydrolase fold | Caps | Esterase | Glycosylated
